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Title

Sulfur assimilation by Oxyrrhis marina feeding on a 35S-DMSP-labelled prey

AuthorsSalo, Violeta CSIC; Simó, Rafel CSIC ORCID ; Vila-Costa, Maria CSIC ORCID; Calbet, Albert CSIC ORCID
Issue DateDec-2009
PublisherWiley-Blackwell
CitationEnvironmental Microbiology 11(12): 3063-3072 (2009)
AbstractA laboratory grazing experiment was conducted with the aim of quantifying the sulfur assimilation by a herbivore protist feeding on a dimethylsulfoniopropionate (DMSP)-containing phytoplankter. When supplied with dissolved 35S-DMSP, cultures of an axenic strain of the diatom Thalassiosira pseudonana took up 60–95% of the added radioisotope and accumulated it untransformed in the cytoplasm. Radiolabelled diatom cells were offered as prey to the heterotrophic dinoflagellate Oxyrrhis marina. After 32 h in the dark, all the prey had been grazed and digested, leaving only radiolabelled O. marina in the grazing bottles and thus providing an estimate of the percentage of DMSP-sulfur retained by the predator. Subsequent precipitation with cold trichloroacetic acid (TCA) provided the fraction of retained DMSP-S that had been assimilated into the micrograzer macromolecules. In parallel incubations with predator and dissolved 35S-DMSP only (no prey), O. marina (and their closely associated bacteria) took up the radiolabelled substrate osmotrophically to an activity of 0.04 dpm cell−1 and assimilated it all into macromolecules. By correcting grazing 35S-DMSP assimilation for osmotrophic 35S-DMSP assimilation, and comparing it with the ingested radioisotope, the percentage of ingested DMSP-sulfur retained and assimilated by the predator was determined to be 32 ± 4%. This is the first study that provides direct evidence that ingestion of a DMSP-containing prey supplies structural sulfur to a herbivore protist and that quantifies this assimilative supply at one-third of ingested DMSP
Description10 pages, 6 figures, 1 table
Publisher version (URL)https://doi.org/10.1111/j.1462-2920.2009.02011.x
URIhttp://hdl.handle.net/10261/19216
DOI10.1111/j.1462-2920.2009.02011.x
ISSN1462-2912
Appears in Collections:(ICM) Artículos

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