Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/192107
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains

AuthorsSilván, José Manuel CSIC ORCID ; Zorraquín-Peña, Irene; González de Llano, Dolores CSIC ORCID ; Moreno-Arribas, M. Victoria CSIC ORCID ; Martínez-Rodríguez, Adolfo J. CSIC ORCID
KeywordsAntibacterial activity
Campylobacter
Silver nanoparticles
Antibiotic resistance
Multidrug resistant strains
Issue Date2018
PublisherFrontiers Media
CitationFrontiers in Microbiology 9: 458 (2018)
AbstractCampylobacter is the leading cause of bacterial diarrheal disease worldwide. Although most episodes of campylobacteriosis are self-limiting, antibiotic treatment is usually needed in patients with serious enteritis, and especially in childrens or the elderly. In the last years, antibiotic resistance in Campylobacter has become a major public health concern and a great interest exists in developing new antimicrobial strategies for reducing the impact of this food-borne pathogen on human health. Among them, the use of silver nanoparticles as antibacterial agents has taken on increased importance in the field of medicine. The aim of the present study was to evaluate the antimicrobial effectiveness of glutathione-stabilized silver nanoparticles (GSH-Ag NPs) against multidrug resistant (MDR) Campylobacter strains isolated from the chicken food chain (FC) and clinical patients (C). The results obtained showed that GSH-Ag NPs were highly effective against all MDR Campylobacter strains tested. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were in a range from 4.92 to 39.4 μg/mL and 9.85 to 39.4 μg/mL, respectively. Cytotoxicity assays were also assessed using human intestinal HT-29, Caco-2, and CCD-18 epithelial cells. Exposure of GSH-Ag NPs to intestinal cells showed a dose-dependent cytotoxic effect in all cell lines between 9.85 and 39.4 μg/mL. More than 60% of the tested Campylobacter strains were susceptible to GSH-Ag NPs concentrations ≤ 9.85 μg/mL, suggesting that practical inhibitory levels could be reached at low GSH-Ag NPs concentrations. Further works are needed with the purpose to evaluate the practical implications of the toxicity studies and to know more about other attributes linked to the biological compatibility. This behavior makes GSH-Ag NPs as a promising tool for the design of novel antibacterial agents for controlling Campylobacter.
Publisher version (URL)https://doi.org/10.3389/fmicb.2018.00458
URIhttp://hdl.handle.net/10261/192107
DOI10.3389/fmicb.2018.00458
Identifiersdoi: 10.3389/fmicb.2018.00458
e-issn: 1664-302X
Appears in Collections:(CIAL) Artículos

Files in This Item:
File Description SizeFormat
antimultiresist.pdf552,69 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

10
checked on Jan 21, 2022

SCOPUSTM   
Citations

23
checked on Jan 17, 2022

WEB OF SCIENCETM
Citations

21
checked on Jan 18, 2022

Page view(s)

294
checked on Jan 23, 2022

Download(s)

96
checked on Jan 23, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.