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dc.contributor.authorGomez-Valero, Lauraes_ES
dc.contributor.authorChiner-Oms, Álvaroes_ES
dc.contributor.authorComas, Iñakies_ES
dc.contributor.authorBuchrieser, Carmenes_ES
dc.date.accessioned2019-10-02T10:33:58Z-
dc.date.available2019-10-02T10:33:58Z-
dc.date.issued2019-09-01-
dc.identifier.citationGenome biology and evolution 11(9):2619-2632 (2019)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/192052-
dc.description14 páginas, 2 figuras, 2 tablases_ES
dc.description.abstractThe Dot/Icm type IVB secretion system of Legionella pneumophila is essential for its pathogenesis by delivering >300 effector proteins into the host cell. However, their precise secretion mechanism and which components interact with the host cell is only partly understood. Here, we undertook evolutionary analyses of the Dot/Icm system of 58 Legionella species to identify those components that interact with the host and/or the substrates. We show that high recombination rates are acting on DotA, DotG, and IcmX, supporting exposure of these proteins to the host. Specific amino acids under positive selection on the periplasmic region of DotF, and the cytoplasmic domain of DotM, support a role of these regions in substrate binding. Diversifying selection acting on the signal peptide of DotC suggests its interaction with the host after cleavage. Positive selection acts on IcmR, IcmQ, and DotL revealing that these components are probably participating in effector recognition and/or translocation. Furthermore, our results predict the participation in host/effector interaction of DotV and IcmF. In contrast, DotB, DotO, most of the core subcomplex elements, and the chaperones IcmS-W show a high degree of conservation and not signs of recombination or positive selection suggesting that these proteins are under strong structural constraints and have an important role in maintaining the architecture/function of the system. Thus, our analyses of recombination and positive selection acting on the Dot/Icm secretion system predicted specific Dot/Icm components and regions implicated in host interaction and/or substrate recognition and translocation, which will guide further functional analyses.es_ES
dc.description.sponsorshipThis work was supported by the Institut Pasteur, the Agence National de la Recherche (grant nANR-10-LABX-62-IBEID) and the Fondation pour la Recherche Medicale (FRM) (grant NDEQ20120323697).es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectLegionellaes_ES
dc.subjectDot/Icm systemes_ES
dc.subjectDiversifying-selectiones_ES
dc.subjectEvolutiones_ES
dc.subjectNegative-selectiones_ES
dc.subjectPositive-selectiones_ES
dc.titleEvolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Specieses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1093/gbe/evz186-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1093/gbe/evz186es_ES
dc.identifier.e-issn1759-6653-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc/4.0/es_ES
dc.contributor.funderInstitut Pasteures_ES
dc.contributor.funderAgence Nationale de la Recherche (France)es_ES
dc.contributor.funderFondation pour la Recherche Médicalees_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001665es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002915es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003762es_ES
dc.contributor.orcidComas, Iñaki [0000-0001-5504-9408]es_ES
dc.contributor.orcidChiner-Oms, Álvaro [0000-0002-0463-0101]es_ES
dc.identifier.pmid31504472-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeartículo-
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