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Title

A bioassay method to determine the insecticidal activity of Bacillus thuringiensis against Ceratitis capitata (Diptera: Tephritidae) and Drosophila suzukii (Diptera: Drosophilidae)

AuthorsValtierra, Daniel ; Matas, Isabel M. ; Caballero, Javier ; Caballero, Primitivo
Issue Date24-Jul-2016
Citation49th Annual Meeting of the Society for Invertebrate Pathology (2016)
AbstractFruit flies are considered to be highly invasive polyphagous pests that cause serious damage to many crops worldwide. In the Mediterranean region, C. capitata (Wiedemann) and D. suzukii (Matsumura) are of major economic importance. Bacillus thuringiensis (Berliner) (Bt) has been considered as a potential source of insecticidal proteins for the control of C. capitata. However, bioassay methods used in previous studies have been hampered due to difficulties of knowing the number of individuals that consumed experimental inocula and issues related to quantifying the dose ingested by adult flies. We aimed to develop a novel, reproducible bioassay method for C. capitata and D. suzukii. This method uses a liquid colorant added to a food substrate that allows the identification of inoculated adults. It was also possible to determine thevolume ingested in order to calculate lethal dose relationships. We demonstrate the value of this bioassay method using Btstrains, and a specific Btprotein (Cry2Ab25),which showed activity against Ragolethis cerasi (Tephritidae)
DescriptionTrabajo presentado en el 49th Annual Meeting of the Society for Invertebrate Pathology: International Congress on Invertebrate Pathology and Microbial Control, celebrado en Tours (Francia), del 24 al 28 de julio de 2016
URIhttp://hdl.handle.net/10261/191933
Appears in Collections:(IDAB) Comunicaciones congresos
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