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dc.contributor.authorZabalza-Baranguá, Anaes_ES
dc.contributor.authorSan Román, Beatrizes_ES
dc.contributor.authorChacón Diaz, Carloses_ES
dc.contributor.authorMiguel, María Jesús dees_ES
dc.contributor.authorMuñoz, Pilar M.es_ES
dc.contributor.authorIriarte, Maitees_ES
dc.contributor.authorBlasco, José M.es_ES
dc.contributor.authorGrilló, María Jesúses_ES
dc.date.accessioned2019-09-30T11:20:47Z-
dc.date.available2019-09-30T11:20:47Z-
dc.date.issued2019-01-
dc.identifier.citationTransboundary and Emerging Diseases 66(1): 505-516 (2019)es_ES
dc.identifier.issn1865-1674-
dc.identifier.urihttp://hdl.handle.net/10261/191863-
dc.description.abstractBrucellosis is a worldwide zoonosis causing important economic loss and a public health problem. Small ruminants are the preferred hosts of Brucella melitensis and thus the main source of human infections. Effective control of sheep and goat brucellosis has been achieved in several countries through vaccination with the live‐attenuated B. melitensis Rev1 vaccine. However, Rev1 induces a long‐lasting serological response that hinders the differentiation between infected and vaccinated animals. A Rev1::gfp strain expressing constitutively the Green Fluorescent Protein (GFP) was built by stable insertion of a mini‐Tn7‐gfp in the glmS‐recG non‐codifying chromosomal region. An associated indirect ELISA‐GFP was developed to identify anti‐GFP antibodies in vaccinated animals. The resulting Rev1::gfp kept the biological properties of the Rev1 reference strain, including residual virulence and efficacy in mice, and was readily distinguished from Rev1 and other Brucella field strains by direct visualization under ultraviolet illumination, fluorescence microscopy and a multiplex PCR‐GFP. The Rev1::gfp strain did not elicit anti‐GFP antibodies itself in lambs but when applied in combination with recombinant GFP induced an intense and long‐lasting (>9 months) anti‐GFP serological response readily detectable by the ELISA‐GFP. Overall, our results confirm that Rev1 GFP‐tagging can be a suitable alternative for identifying vaccinated sheep in infected contexts.es_ES
dc.description.sponsorshipSecretaría de Estado de Investigación,Desarrollo e Innovación, Grant/AwardNumber: AGL2011-30453-C04-01,AGL2014-58795-C04, RTC-2015-3618-1;CSIC-CRUSA Foundation bilateral project,Grant/Award Number: 2010CR0005es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-58795-C04es_ES
dc.relationMINECO/ICTI2013-2016/RTC-2015-3618-1es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectBrucella melitensises_ES
dc.subjectELISA‐GFPes_ES
dc.subjectMini‐Tn7‐gfpes_ES
dc.subjectSheepes_ES
dc.subjectVaccineses_ES
dc.titleGFP tagging of Brucella melitensis Rev1 allows the identification of vaccinated sheepes_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1111/tbed.13053-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1111/tbed.13053es_ES
dc.identifier.e-issn1865-1682-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.contributor.funderFundación CRUSAes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.contributor.orcidGrilló, María Jesús [0000-0002-1819-0186]es_ES
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