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Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis

AuthorsAmeztoy, Kinia CSIC ORCID; Baslam, Marouane CSIC ORCID; Sánchez-López, Ángela María CSIC ORCID ; Muñoz Pérez, Francisco José CSIC ORCID ; Bahaji, Abdellatif CSIC ORCID ; Almagro, Goizeder CSIC ORCID ; García-Gómez, Pablo CSIC ORCID; Baroja-Fernández, Edurne CSIC ORCID CVN ; Diego, Nuria de; Humplík, Jan F.; Ugena, Lydia; Spíchal, Lukáš; Doležal, Karel; Kaneko, Kentaro; Mitsui, Toshiaki; Cejudo, Francisco Javier CSIC ORCID; Pozueta Romero, Javier CSIC ORCID
KeywordsGrowth promotion
Hormone signalling
Microbial volatile compounds
Plant–microbe interactions
Redox proteomics
Issue DateSep-2019
PublisherJohn Wiley & Sons
CitationPlant Cell and Environment 42(9): 2627-2644 (2019)
AbstractMicroorganisms produce volatile compounds (VCs) that promote plant growth and photosynthesis through complex mechanisms involving cytokinin (CK) and abscisic acid (ABA). We hypothesized that plants' responses to microbial VCs involve posttranslational modifications of the thiol redox proteome through action of plastidial NADPH‐dependent thioredoxin reductase C (NTRC), which regulates chloroplast redox status via its functional relationship with 2‐Cys peroxiredoxins. To test this hypothesis, we analysed developmental, metabolic, hormonal, genetic, and redox proteomic responses of wild‐type (WT) plants and a NTRC knockout mutant (ntrc) to VCs emitted by the phytopathogen Alternaria alternata. Fungal VC‐promoted growth, changes in root architecture, shifts in expression of VC‐responsive CK‐ and ABA‐regulated genes, and increases in photosynthetic capacity were substantially weaker in ntrc plants than in WT plants. As in WT plants, fungal VCs strongly promoted growth, chlorophyll accumulation, and photosynthesis in ntrc–Δ2cp plants with reduced 2‐Cys peroxiredoxin expression. OxiTRAQ‐based quantitative and site‐specific redox proteomic analyses revealed that VCs promote global reduction of the thiol redox proteome (especially of photosynthesis‐related proteins) of WT leaves but its oxidation in ntrc leaves. Our findings show that NTRC is an important mediator of plant responses to microbial VCs through mechanisms involving global thiol redox proteome changes that affect photosynthesis.
Publisher version (URL)https://doi.org/10.1111/pce.13601
Appears in Collections:(IDAB) Artículos
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