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Title

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

AuthorsInomata, Takuya; Baslam, Marouane ; Masui, Takahiro; Koshu, Tsutomu; Takamatsu, Takeshi; Kaneko, Kentaro; Pozueta Romero, Javier ; Mitsui, Toshiaki
KeywordsChloroplast
Elevated CO2
Heat stress
Nucleotide pyrophosphatase/phosphodiesterase
(phospho)-proteomics
Photosynthesis
Protein phosphorylation
14-3-3 proteins
Oryza sativa L.
Starch
Sucrose
Issue Date7-Sep-2018
PublisherMolecular Diversity Preservation International
CitationInternational Journal of Molecular Sciences 19(9): 2655 (2018)
AbstractRice nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides including ADP-glucose and ATP. Under high temperature and elevated CO2 conditions (HT + ECO2), the npp1 knockout rice mutant displayed rapid growth and high starch content phenotypes, indicating that NPP1 exerts a negative effect on starch accumulation and growth. To gain further insight into the mechanisms involved in the NPP1 downregulation induced starch overaccumulation, in this study we conducted photosynthesis, leaf proteomic, and chloroplast phosphoproteomic analyses of wild-type (WT) and npp1 plants cultured under HT + ECO2. Photosynthesis in npp1 leaves was significantly higher than in WT. Additionally, npp1 leaves accumulated higher levels of sucrose than WT. The proteomic analyses revealed upregulation of proteins related to carbohydrate metabolism and the protein synthesis system in npp1 plants. Further, our data indicate the induction of 14-3-3 proteins in npp1 plants. Our finding demonstrates a higher level of protein phosphorylation in npp1 chloroplasts, which may play an important role in carbohydrate accumulation. Together, these results offer novel targets and provide additional insights into carbohydrate metabolism regulation under ambient and adverse conditions.
Publisher version (URL)https://doi.org/10.3390/ijms19092655
URIhttp://hdl.handle.net/10261/191622
DOI10.3390/ijms19092655
ISSN1661-6596
E-ISSN1422-0067
Appears in Collections:(IDAB) Artículos
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