English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/191523
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Characterization and nutritional regulation of ghrelin and its receptors in Gilthead sea bream (Sparus aurata)

AuthorsPerelló-Amorós, Miguel; Vélez, Emilio ; Vela-Albesa, Jaume; Sánchez-Moya, Albert; Riera-Heredia, Natàlia; Hedén, Ida; Fernández-Borràs, Jaume; Blasco, Josefina; Calduch-Giner, Josep A. ; Navarro, Isabel; Capilla, E.; Jönsson, Elisabeth; Pérez-Sánchez, Jaume ; Gutiérrez, Joaquim
Issue Date18-Aug-2018
Citation29th CECE (2018)
AbstractGhrelin is a peptide hormone that regulates different physiological processes in vertebrates (feeding, metabolism, intestinal activity and growth hormone (Gh) secretion). However, specific actions of this hormone have been reported to change among species. The objective of this work was to characterize preproghrelin and Ghrelin receptors (ghsrs) sequences, and to study their responses to fasting and refeeding in gilthead sea bream (Sparus aurata) juveniles. Structural and phylogenetic analysis of the S. aurata preproghrelin was accomplished, and its expression analyzed in main tissues. Fish were fasted for 21 days and then refed and sampled at 2, 5, 24 h, and 7 days afterwards. Plasma levels of Ghrelin, Gh and insulin-like growth factor (Igf-1), and preproghrelin, ghsrs and members of the Gh/Igf-1 axis gene expression were determined in key tissues. preproghrelin and its receptors presented a good conservation among fish, being highly expressed in stomach and pyloric caeca, and in the pituitary and brain, respectively. Fasting caused a growth recession while increased Ghrelin plasma levels, which rapidly recovered at 5 h postprandial. Gh plasma levels were also increased by fasting, and slowly recovered after refeeding. Contrarily, circulant Igf-1 followed an inverse pattern, suggesting important changes in the Gh/Igf ratio by nutritional status. In agreement with the plasma levels, fasting up-regulated gh expression in pituitary until 1 day after refeeding, when lowest igf-1 expression in the liver was observed. Moreover, the expression profile of hepatic Gh receptors resulted to be inverse to that if circulating Gh levels. Altogether, this study demonstrates the important role of Ghrelin concerning nutritional status, suggesting an orexigenic function in gilthead sea bream, and an interaction with pituitary Ghsrs and Gh/Igf-1 axis to promote optimal growth in this species
DescriptionTrabajo presentado en el 29th Conference of European Comparative Endocrinologists (CECE), celebrado en Glasgow, del 19 al 22 de agosto de 2018
Appears in Collections:(IATS) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,35 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.