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Humoral response of gilthead sea bream (Sparus aurata) rechallenged with the intestinal parasite Enteromyxum leei

AuthorsPicard-Sánchez, Amparo; Piazzon de Haro, María Carla ; Estensoro, Itziar ; Pozo, R. del; Calduch-Giner, Josep A. ; Pérez-Sánchez, Jaume ; Palenzuela, Oswaldo ; Sitjà-Bobadilla, Ariadna
Issue Date29-Apr-2018
Citation19th Fish Immunology Workshop (2018)
AbstractThe intestinal myxosporean parasite Enteromyxum leei causes chronic catarrhal enteritis in gilthead sea bream (GSB, Sparus aurata) leading to intestinal dysfunction, poor growth, and higher susceptibility to handling and stress. It entails severe economic losses to the aquaculture sector. Previous observations in our lab showed that fish that recovered from an E. leei infection did not get infected upon re-exposure, hinting towards the possibility of protective immunization of GSB against E. leei. To study this in more detail, we rechallenged putative ¿resistant¿ (R) GSB that recovered from this myxozoan infection, by exposure to E. leei effluent infected water. Another group of naïve (N) GSB (never exposed to the parasite) was also challenged. We sampled the fish at 0, 61 and 86 days post-exposure (p.e.) and we measured different specific and non-specific humoral factors in R and N fish, such as the total peroxidase activity, total IgM and IgT (by ELISA), and presence and quantity of specific antibodies (Abs) against the parasite (by IHC). We also studied the expression of a panel of immune-related genes in head kidney, anterior and posterior intestine of N and R fish after 86 days p.e. Total peroxidase activity decreased with the progression of the infection in both groups, but only significantly in R fish, probably due to a depletion of granulocytes that is a known effect of this parasite. Total IgT and IgM levels did not significantly differ between groups. However, IHC evidenced that R fish had a higher initial level of specific Abs than N fish. Furthermore, at the final sampling point, 83.3% of N fish vs none of the R fish, tested positive for E. leei by qPCR. This higher initial pool of specific Abs against the parasite could be responsible for the observed protection. Hence, this acquired protective immunity sets the grounds for the development of a vaccine or to produce recombinant antibodies against E. leei.
DescriptionTrabajo presentado en el 19th Fish Immunology Workshop, celebrado en Wageningen (Holanda), del 29 de abril al 3 de mayo de 2018
Appears in Collections:(IATS) Comunicaciones congresos
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