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T cell response in gilthead sea bream after exposure to the myxozoan parasite Enteromyxum leei: Local versus systemic

AuthorsPiazzon de Haro, María Carla ; Pozo, R. del; Calduch-Giner, Josep A. ; Picard-Sánchez, Amparo; Estensoro, Itziar ; Pérez-Sánchez, Jaume ; Sitjà-Bobadilla, Ariadna
Issue Date4-Sep-2017
Citation18th International Conference on Diseases of Fish and Shellfish (2017)
AbstractThe study of T cell responses in fish has been hampered by the lack of specific antibodies and molecular tools. Nowadays, several T cell specific antibodies have been developed for a few fish species. However, our knowledge on fish T cell responses is mainly derived from gene expression analyses of signature genes characterized as a result of the recent availability of genomic and transcriptomic information. Enteromyxum leei is a myxozoan intestinal parasite that produces a slow-progressing intestinal disease leading to anorexia, cachexia and mortalities. This parasite invades the paracellular space of the intestinal epithelium and progresses from the posterior to the anterior intestinal segment. In this study, we define the T cell populations in different intestinal segments and in immune relevant tissues of gilthead sea bream after E. leei infection by anal inoculation. Data mining of the gilthead sea bream transcriptomic database (Nutrigroup-IATS) allowed the construction of a PCR-array composed of 20 different T cell signature transcription factors, cytokines and effector molecules. Our results show that upon E. leei infection, the transcription of the pan T cell markers zap70 and cd3¿, the helper T cell (Th) specific cd4 and the cytotoxic T cell (Tc) specific cd8¿ and cd8ß decreased in head kidney and increased in anterior intestine. The parasite induced a shift from a Th2 to a Th1 response in infected anterior intestines, characterized by down-regulation of the transcription factor gata3 and up-regulation of t-bet, respectively. Significant up-regulation of gzmA and prf1 expression also supported the increase of Tc cells in infected intestines. However, the posterior intestine showed a mixed profile skewed to the anti-inflammatory side, characterized by up-regulation of foxp3, gata3, il10 and il6, still with significant presence of t-bet and ifn¿ transcripts. The expression of il17A/F was significantly up-regulated in infected posterior intestines. Analyses of the overall expression of these signature molecules separated groups of fish by their infection status. In conclusion, E. leei infection activated different T cell phenotypes depending on the intestinal segment and the intensity of infection. This could be due to the chronology of the parasite establishment along the intestinal segments.
DescriptionTrabajo presentado en la 18th International Conference on Diseases of Fish and Shellfish, celebrada en Belfast, del 4 al 8 de septiembre de 2017
Appears in Collections:(IATS) Comunicaciones congresos
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