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Título: | Reduced graphene oxide-carboxymethylcellulose layered with platinum nanoparticles/PAMAM dendrimer/magnetic nanoparticles hybrids. Application to the preparation of enzyme electrochemical biosensors |
Autor: | Borisova, Boryana; Sánchez, Alfredo; Jiménez-Falcao, Sandra; Martín, Miriam; Salazar, Pedro CSIC ORCID CVN; Parrado, Concepción; Pingarrón, José Manuel; Villalonga, Reynaldo | Palabras clave: | Graphene Hybrid nanomaterials Magnetic nanoparticles Biosensors Fish freshness |
Fecha de publicación: | sep-2016 | Editor: | Elsevier | Citación: | Sensors and Actuators - B - Chemical Biochemical Sensors 232: 84-90 (2016) | Resumen: | The assembly of a novel layer-by-layer biosensor architecture using hybrid nanomaterials is explored for the construction of an amperometric enzyme biosensors. The nanostructured sensing interface was prepared with poly(dopamine)-modified magnetic nanoparticles which were covalently coated with four-generation ethylenediamine core polyamidoamine G-4 dendrimers and further decorated with platinum nanoparticles. This nanohybrid was fully characterized and further layered on glassy carbon electrodes coated with a graphene oxide-carboxymethylcellulose hybrid nanomaterial through electrostatic interactions. The nanostructured surface was then employed as scaffold for the covalent immobilization of the enzyme xanthine oxidase through a glutaraldehyde-mediated cross-linking. The enzyme electrode allowed the amperometric detection of xanthine in the 50 nM-12 μM range, with a high sensitivity of 140 mA/M cm and low detection limit of 13 nM. The biosensor exhibited high reproducibility and repeatability, and was successfully tested for the quantification of xanthine in fish samples. | Versión del editor: | https://doi.org/10.1016/j.snb.2016.02.106 | URI: | http://hdl.handle.net/10261/191120 | DOI: | 10.1016/j.snb.2016.02.106 | ISSN: | 0925-4005 |
Aparece en las colecciones: | (ICMS) Artículos |
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