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http://hdl.handle.net/10261/191114
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Salazar, P. | - |
dc.contributor.author | Martín, M. | - |
dc.contributor.author | O'Neill, R. D. | - |
dc.contributor.author | González-Mora, José Luis | - |
dc.date.accessioned | 2019-09-20T07:35:59Z | - |
dc.date.available | 2019-09-20T07:35:59Z | - |
dc.date.issued | 2016-11-01 | - |
dc.identifier.citation | Sensors and Actuators - B - Chemical Biochemical Sensors 235: 117-125 (2016) | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10261/191114 | - |
dc.description.abstract | Herein we report a Prussian Blue modified carbon fiber electrode (CFE/PB) to be used in microbiosensors for glutamate monitoring in physiological applications as an alternative to the classical Pt and Pt-Ir transducers. Their low dimensions (∼250 μm CFE length and ∼10 μm diameter) are advantageous for measuring in living tissues. In addition, PB-modified microelectrodes allow the detection of enzyme-generated hydrogen peroxide at a low applied potential (∼0.0 V against SCE), contrasting the high potential used in many previous designs (∼0.7 V), decreasing the endogenous interference contributions. Moreover, the electrosynthesized polymer, poly-o-phenylenediamine (PoPD), was used to improve biosensor stability and selectivity. CFE/PB was conveniently characterized using impedance, Raman and XPS spectroscopies. Optimization of the fabrication procedure and analytical conditions is described, including activation of CFE/PB, enzyme enrichment, cross-linking, stabilization and anti-interference. A range of analytical parameters were also characterized such as sensitivity, limit of detection, linear range, and enzymatic loading. Finally, an optimized biosensor displaying a linear sensitivity of 135 ± 2 nA μM cm (n = 3), LOD of <2 μM, linear range up to 150 μM and effectively free of interference, is proposed as a suitable candidate for in-vivo glutamate monitoring in the central nervous system. | - |
dc.description.sponsorship | This research was supported by the grant INNPACTO-MINECO (IPT-2012-0961-300000), Ministerio de Ciencia e Innovación (TIN2011-28146), project RECUPERA 2020 from MINECO and the “Fondo social Europeo”, project MAT2013-40852-R and MAT2013-42900-P from MINECO and TEP8067 and FQM6900 from the Junta de Andalucía. | - |
dc.publisher | Elsevier | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2013-40852-R | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2013-42900-P | - |
dc.rights | closedAccess | - |
dc.subject | Biosensors | - |
dc.subject | Glutamate | - |
dc.subject | Prussian blue | - |
dc.subject | Carbon fiber electrode | - |
dc.subject | Poly-o-phenylenediamine | - |
dc.subject | Neurochemistry | - |
dc.title | Glutamate microbiosensors based on Prussian blue modified carbon fiber electrodes for neuroscience applications: in-vitro characterization | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.snb.2016.05.057 | - |
dc.relation.publisherversion | https://doi.org/10.1016/j.snb.2016.05.057 | - |
dc.date.updated | 2019-09-20T07:35:59Z | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
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