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Título: | Viability of endolithic micro-organisms in rocks from the McMurdo Dry Valleys of Antarctica established by confocal and fluorescence microscopy |
Autor: | Wierzchos, Jacek CSIC ORCID ; Ríos, Asunción de los CSIC ORCID ; Sancho, Leopoldo G.; Ascaso, Carmen CSIC ORCID | Palabras clave: | Antarctica Confocal microscopy Endolithic micro-organisms Fluorescence Live/dead assay Viability |
Fecha de publicación: | 2004 | Editor: | Royal Microscopical Society (Great Britain) | Citación: | Journal of Microscopy 216:57-61(2004) | Resumen: | The rocks of the McMurdo Dry Valleys desert in Antarctica harbour endolithic communities of micro-organisms such as lichens, fungi, cyanobacteria and bacteria. Establishing the physiological status and viability of these microbial colonies in their natural microhabitat has far-reaching implications for understanding the microbial ecology of the harsh environment of this polar desert. Here we describe the use of confocal microscopy and a specific fluorescent probe (FUN-1) to evaluate the metabolic activity of fungal cells. Application of confocal microscopy also served to identify living and dead bacteria or cyanobacteria using the fluorescent assay reagents Live/Dead SYTO 9 and propidium iodide or SYTOX Green, respectively. In addition, through the use of epifluorescence microscopy, live/dead bacteria and cyanobacteria could be detected by estimating fluorescence from their cell components provoked by simultaneously staining with nucleic acids stains such as DAPI and SYTOX Green. | Descripción: | 5 pages, and 1 figure. | URI: | http://hdl.handle.net/10261/19111 | DOI: | 10.1111/j.0022-2720.2004.01386.x | ISSN: | 0022-2720 |
Aparece en las colecciones: | (IRN) Artículos |
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