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Título

Viability of endolithic micro-organisms in rocks from the McMurdo Dry Valleys of Antarctica established by confocal and fluorescence microscopy

AutorWierzchos, Jacek ; Ríos, Asunción de los ; Sancho, Leopoldo G.; Ascaso, Carmen
Palabras claveAntarctica
Confocal microscopy
Endolithic micro-organisms
Fluorescence
Live/dead assay
Viability
Fecha de publicación2004
EditorRoyal Microscopical Society (Great Britain)
CitaciónJournal of Microscopy 216:57-61(2004)
ResumenThe rocks of the McMurdo Dry Valleys desert in Antarctica harbour endolithic communities of micro-organisms such as lichens, fungi, cyanobacteria and bacteria. Establishing the physiological status and viability of these microbial colonies in their natural microhabitat has far-reaching implications for understanding the microbial ecology of the harsh environment of this polar desert. Here we describe the use of confocal microscopy and a specific fluorescent probe (FUN-1) to evaluate the metabolic activity of fungal cells. Application of confocal microscopy also served to identify living and dead bacteria or cyanobacteria using the fluorescent assay reagents Live/Dead SYTO 9 and propidium iodide or SYTOX Green, respectively. In addition, through the use of epifluorescence microscopy, live/dead bacteria and cyanobacteria could be detected by estimating fluorescence from their cell components provoked by simultaneously staining with nucleic acids stains such as DAPI and SYTOX Green.
Descripción5 pages, and 1 figure.
URIhttp://hdl.handle.net/10261/19111
DOIhttp://dx.doi.org/10.1111/j.0022-2720.2004.01386.x
ISSN0022-2720
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