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Population genetics and transcriptomics of Manila Clam (Ruditapes philippinarum) and carpet-shell clam (R. decussatus): implications for aquaculture

AuthorsSaavedra, Carlos Felipe CSIC ORCID; Milan, Massimo; Cordero, David CSIC; Leite, Ricardo B.; Peña, Juan B. CSIC; Delgado, María Jesús; Liu, Baozhong; Ruesink, J.; Cancela, M. Leonor; Bargelloni, Luca; Patarnello, Tomaso
Mitochondrial DNA
Issue Date21-Jun-2015
CitationXII ISGA (2015)
AbstractKnowledge of the genetics of populations is essential to understand performance differences among races and local stocks of shellfish species. We have studied the population genetics of the native Asian -but now globally cultured- Manila clam, and the native European carpet-shell clam, which are exploited in Europe by a combination of wild bed harvesting and supplementation with hatchery spat. Using mtDNA and nuclear markers we found that both species show clear phylogeographic breaks at the Mediterranean Sea (carpet-shell clam) and at the East China Sea (Manila clam). Moreover, we confirmed a Japanese origin for American and European Manila clam populations, which in spite of the short time interval passed since the introduction, have experienced a considerable genetic differentiation, and some loss of genetic variability in Europe. To study the potential functional significance of phylogeographic differences we used a transcriptomic approach in R. decussatus. We studied the gene expression in the gill tissue of clams from four populations sampled along the north-south thermal gradients of Atlantic and west Mediterranean European coasts with a microarray containing 14003 oligonucleotide probes. Clams were acclimated for three months in a common environment before the experiment. Using a multiple regression approach we found that a model based on the latitude and longitude of the populations, an index of phylogeographic differentiation (based on 3 polymorphic introns), and their interactions, explained a considerable fraction of the interindividual variation in gene expression for 1706 genes (FDR = 0.22). Functional characterization uncovered an abundance of stress-related genes in the group of differentially expressed genes. These results imply that the choice of the geographic origin of clam spat or breeders could have important consequences on the results of population supplementation or breeding programs.
DescriptionTrabajo presentado en el XII International Symposium on Genetics in Aquaculture (ISGA), celebrado en Santiago de Compostela (España) del 21 al 27 de junio de 2015
Appears in Collections:(IATS) Comunicaciones congresos
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