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http://hdl.handle.net/10261/19039
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dc.contributor.author | Pinheiro, C. B. | - |
dc.contributor.author | Marangoni, S. | - |
dc.contributor.author | Toyama, M. H. | - |
dc.contributor.author | Polikarpov, I. | - |
dc.date.accessioned | 2009-11-26T09:34:29Z | - |
dc.date.available | 2009-11-26T09:34:29Z | - |
dc.date.issued | 2003-03 | - |
dc.identifier.citation | Acta Crystallographica Section D 59(3): 405-415 (2003) | en_US |
dc.identifier.issn | 1067-0696 | - |
dc.identifier.uri | http://hdl.handle.net/10261/19039 | - |
dc.description | 11 pages, 7 figures, 2 tables. | en_US |
dc.description.abstract | The structure of the Ts1 toxin from the Brazilian scorpion Tityus serrulatus was investigated at atomic resolution using X-ray crystallography. Several positively charged niches exist on the Ts1 molecular surface, two of which were found to coordinate phosphate ions present in the crystallization solution. One phosphate ion is bound to the conserved basic Lys1 residue at the Ts1 N-terminus and to residue Asn49. The second ion was found to be caged by residues Lys12, Trp54 and Arg56. Lys12 and Tyr/Trp54 residues are strictly conserved in all classical scorpion [beta]-neurotoxins. The cavity formed by these residues may represent a special scaffold required for interaction between [beta]-neurotoxins and sodium channels. The charge distribution on the Ts1 surface and the results of earlier chemical modification studies and side-directed mutagenesis experiments strongly indicate that the phosphate-ion positions mark plausible binding sites to the Na+ channel. The existence of two distinct binding sites on the Ts1 molecular surface provides an explanation for the competition between Ts1, depressant (LqhIT2) and excitatory (AaHIT) neurotoxins. | en_US |
dc.description.sponsorship | This work was supported by the FAPESP (FundacËaÄo de Amparo aÁ Pesquisa do Estado de SaÄo Paulo) grants 99/08042-5 and 99/03387-4.We thank Professor Oussama Hassani for kindly providing us with the coordinates of the Ts1 neurotoxin obtained by comparative modelling and the LNLS personnel for giving us access to the synchrotron-facility infrastructure. | en_US |
dc.format.extent | 13824 bytes | - |
dc.format.mimetype | application/vnd.ms-excel | - |
dc.language.iso | eng | en_US |
dc.publisher | International Union of Crystallography | en_US |
dc.rights | openAccess | en_US |
dc.subject | Scorpion neurotoxins | en_US |
dc.subject | Tityus serrulatus | en_US |
dc.subject | Sodium channels | en_US |
dc.title | Structural analysis of Tityus serrulatus Ts1 neurotoxin at atomic resolution: insights into interactions with Na+ channels | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1107/S090744490202111X | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1107/S090744490202111X | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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