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dc.contributor.authorPrieto, Ignacio-
dc.contributor.authorZambrano, Alberto-
dc.contributor.authorLaso, Javier-
dc.contributor.authorAranda, Ana-
dc.contributor.authorSamper, Enrique-
dc.contributor.authorMonsalve, María-
dc.date.accessioned2019-09-09T12:21:35Z-
dc.date.available2019-09-09T12:21:35Z-
dc.date.issued2018-
dc.identifier.citation19th Biennial Meeting for the Society for Free Radical Research International (2018)-
dc.identifier.urihttp://hdl.handle.net/10261/190258-
dc.descriptionResumen del póster presentado al 19th Biennial Meeting for the Society for Free Radical Research International (SFRRI), celebrado en Lisboa (Portugal) del 4 al 7 de junio de 2018.-
dc.description.abstract[Aims] Oxidative stress is known to induce early replicative senescence.Senescence has been proposed to work as a barrier to immortalizationand tumor development. Here, we aimed to evaluate the impact of theloss of peroxisome proliferator activated receptorγco-activator 1α(PGC-1α), a master regulator of oxidative metabolism and mitochondrial re-active oxygen species (ROS) generation, on replicative senescence andimmortalization in mouse embryonicfibroblasts (MEFs). [Results] We found that primary MEFs lacking PGC-1αshowed higher levelsof ROS than wild-type MEFs at all cell passages tested. The elevated pro-duction of ROS was associated with higher levels of oxidative DNA damageand the increased formation of DNA double-strand breaks. Evaluation ofthe induction of DNA repair systems in response toγ-radiation indicatedthat the loss of PGC-1αalso resulted in a small but significant reduction intheir activity. DNA damage induced the early activation of senescencemarkers, including an increase in the number ofβ-galactosidase-positivecells, the induction of p53 phosphorylation, and the increase in p16 and p19protein. These changes were, however, not sufficient to reduce proliferationrates of PGC-1α-deficient MEFs at any cell passage tested.-
dc.rightsclosedAccess-
dc.titleEarly induction of senescence and immortalization in PGC-1α-deficient mouse embryonic fibroblasts-
dc.typepóster de congreso-
dc.date.updated2019-09-09T12:21:35Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.relation.csic-
Appears in Collections:(IIBM) Comunicaciones congresos
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