Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/190173
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The NADPH-Dependent thioredoxin reductase C-2-Cys peroxiredoxin redox system modulates the activity of thioredoxin x in arabidopsis chloroplasts |
Autor: | Ojeda, Valle; Pérez-Ruiz, Juan Manuel CSIC ; Cejudo, Francisco Javier CSIC ORCID | Palabras clave: | Arabidopsis Peroxiredoxin Redox regulation Thioredoxin Chloroplast |
Fecha de publicación: | oct-2018 | Editor: | Oxford University Press | Citación: | Plant and Cell Physiology 59(10): 2155-2164 (2018) | Resumen: | The chloroplast redox network is composed of a complex set of thioredoxins (Trxs), reduced by ferredoxin (Fdx) via a Fdx-dependent Trx reductase (FTR), and an NADPH-dependent Trx reductase with a joint Trx domain, NTRC, which efficiently reduces 2-Cys peroxiredoxins (2-Cys Prxs). Recently, it was proposed that the redox balance of 2-Cys Prxs maintains the redox state of f-type Trxs, thus allowing the proper redox regulation of Calvin-Benson cycle enzymes such as fructose 1,6-bisphosphatase (FBPase). Here, we have addressed whether the action of 2-Cys Prxs is also exerted on Trx x. To that end, an Arabidopsis thaliana quadruple mutant, ntrc-trxx-δ >2cp, which is knocked out for NTRC and Trx x, and contains severely decreased levels of 2-Cys Prxs, was generated. In contrast to ntrc-trxx, which showed a severe growth inhibition phenotype and poor photosynthetic performance, the ntrc-trxx-δ >2cp mutant showed a significant recovery of growth rate and photosynthetic efficiency, indicating that the content of 2-Cys Prxs is critical for the performance of plants lacking both NTRC and Trx x. Light-dependent reduction of FBPase was severely impaired in mutant plants lacking NTRC or NTRC plus Trx x, despite the fact that neither NTRC nor Trx x is an effective reductant of this enzyme. However, FBPase reduction was recovered in the ntrc-trxx-δ >2cp mutant. Our results show that the redox balance of 2-Cys Prxs, which is mostly dependent on NTRC, modulates the activity of Trx x in a similar way as f-type Trxs, thus suggesting that the activity of these Trxs is highly interconnected. | Versión del editor: | https://doi.org/10.1093/pcp/pcy134 | URI: | http://hdl.handle.net/10261/190173 | DOI: | 10.1093/pcp/pcy134 | ISSN: | 0032-0781 | E-ISSN: | 1471-9053 |
Aparece en las colecciones: | (IBVF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
21
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
20
checked on 21-feb-2024
Page view(s)
162
checked on 23-abr-2024
Download(s)
30
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.