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Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata)

AuthorsGil-Solsona, Ruben; Nácher-Mestre, Jaime; Lacalle-Bergeron, Leticia; Sancho, Juan Vicente; Hernández Hernández, Félix; Pérez-Sánchez, Jaume CSIC ORCID
Mass spectrometry
Gilthead sea bream
Serum metabolomics
Issue Date1-Feb-2017
CitationPeerJ 5:e2920 (2017)
AbstractA metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-offlight mass spectrometry. More than 15,000 different m/z ions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitinerelated metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish.
Publisher version (URL)http://dx.doi.org/10.7717/peerj.2920
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