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Birth of a photosynthetic chassis: a MoClo toolkit enabling synthetic biology in the microalga chlamydomonas reinhardtii

AuthorsCrozet, Pierre; Navarro, Francisco J.; Willmund, Felix; Mehrshahi, Payam; Bakowski, Kamil; Lauersen, Kyle J.; Pérez-Pérez, María Esther CSIC ORCID ; Auroy, Pascaline; Gorchs Rovira, Aleix; Sauret-Gueto, Susana; Niemeyer, Justus; Spaniol, Benjamin; Theis, Jasmine; Trösch, Raphael; Westrich, Lisa-Desiree; Vavitsas, Konstantinos; Baier, Thomas; Hübner, Wolfgang; Carpentier, Felix de; Cassarini, Mathieu; Danon, Antoine; Henri, Julien; Marchand, Christophe H.; Mia, Marcello de; Sarkissian, Kevin; Baulcombe, David C.; Peltier, Gilles; Crespo, José L. CSIC ORCID ; Kruse, Olaf; Jensen, Poul-Erik; Schroda, Michael; Smith, Alison G.; Lemaire, Stéphane D.
KeywordsAlgal biotechnology
Modular cloning
Synthetic biology
Chlamydomonas reinhardtii
Issue Date21-Sep-2018
PublisherAmerican Chemical Society
CitationACS Synthetic Biology 7(9): 2074-2086 (2018)
AbstractMicroalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.
Publisher version (URL)https://doi.org/10.1021/acssynbio.8b00251
Appears in Collections:(IBVF) Artículos
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