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Título: | Vochysia rufa stem bark extract protects endothelial cells against high glucose damage |
Autor: | Moura de Gouveia, Neire; Ramos, Sonia CSIC ORCID ; Martín, M. Ángeles CSIC ORCID ; Salmen Espindola, Foued; Goya, Luis CSIC ORCID; Palomino, Olga | Palabras clave: | Vochysia rufa Sapotaceae Endothelial cells Oxidative damage Plant antioxidants |
Fecha de publicación: | 2017 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Medicines 4(1): 9 (2017) | Resumen: | [Background]: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. [Methods]: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups. [Results]: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg/mL Vr significantly reduced cell damage while 5–25 μg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. [Conclusion]: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels. |
Descripción: | This article belongs to the Special Issue Herbal Medicines and Functional Foods. | Versión del editor: | https://doi.org/10.3390/medicines4010009 | URI: | http://hdl.handle.net/10261/189740 | DOI: | 10.3390/medicines4010009 | E-ISSN: | 2305-6320 |
Aparece en las colecciones: | (ICTAN) Artículos |
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