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| Título: | Engineering of Bacillus megaterium for improving PHA production from glycerol |
Autor: | Gómez Cardozo, Javier Ricardo; Velasco-Bucheli, Rodrigo; Del Cerro, Carlos CSIC ORCID; Mata, Isabel de la; Mora Martínez, Amanda Lucía | Palabras clave: | Bacillus megaterium Glycerol Glycerol dehydrogenase Polyhydroxyalkanoates |
Fecha de publicación: | 2019 | Editor: | Malaysian Society of Molecular Biology & Biotechnology | Citación: | AsPac J Mol Biol Biotechnol 27 (3) : 64-72 (2019) | Resumen: | There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h. | Descripción: | 9 p.-4 fig. | Versión del editor: | https://doi.org/10.35118/apjmbb.2019.027.3.07 | URI: | http://hdl.handle.net/10261/189298 | DOI: | 10.35118/apjmbb.2019.027.3.07 | ISSN: | 0128-7451 | E-ISSN: | 2672-7277 |
| Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Asia-Pacific Journal of Molecular Biology and Biotechnology_Gomez_2019.pdf | Artículo principal | 264,63 kB | Adobe PDF |  Visualizar/Abrir |
| Gomez-et-al-_Supplemental-Material_2019.pdf | Material suplementario | 443,45 kB | Adobe PDF |  Visualizar/Abrir |
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