English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/18902
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Localization of coat protein and nucleic acid of Plum Pox Virus in Prunus Petiole and stem tissues

AuthorsRubio, Manuel ; Gosálvez, Blanca ; Hernández, José Antonio ; Dicenta, Federico ; Martínez-Gómez, Pedro ; Sánchez-Navarro, J. A. ; Pallás Benet, Vicente
KeywordsPrunus
Plum pox virus
Virus
Immuno-tissue printing
Issue Date2004
PublisherInternational Society for Horticultural Science
CitationActa Horticulturae 657: 295-300 (2004)
AbstractLocalization of Plum pox virus (PPV) in stem and petiole tissues of several Prunus species, including apricot, peach, prune, plum, almond, and cherry was studied. In addition, symptom severity and the optical density by ELISA-DASI were determined on leaves. Consecutive 2-mm spaced transverse sections were taken from stem and petiole and were printed onto nitrocellulose (for coat protein detection) and nylon (for RNA detection) membranes. Nylon membranes were reveled by chemiluminescent and colorimetric detection. Distribution of PPV (coat protein and RNA) was very irregular in stem and petiole tissues in apricot, peach, prune and plum, showing PPV symptoms and being ELISA positive. PPV was not localized in the asymptomatic plants (cherry and almond) with negative ELISA reaction. A high correlation was observed between coat protein and RNA localization in the stem and petiole tissues. Despite the high sensitivity of the chemiluminescent procedure, the colorigenic substract allowed the clearly detection of PPV in infected stem and petiole tissues. In the stem, PPV (coat protein and RNA) was mainly localized in vascular tissues and sometimes in cortex, pith and epidermis. In the petiole, it was localized in the epidermis and in cortical and medullae parenchyma. The irregular distribution of the virus, as regards both coat protein and RNA, has negative implications for PPV resistance evaluation, since it renders virus detection.
DescriptionEn: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases, Valencia, (Spain). Editor: G. Llácer.
Publisher version (URL)http://www.actahort.org/books/657/657_45.htm
URIhttp://hdl.handle.net/10261/18902
ISBN978-90-66053-27-4
ISSN0567-7572
Appears in Collections:(CEBAS) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.