English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/188943
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes

AuthorsGuijas, Carlos ; Bermúdez, Miguel A.; Meana, Clara ; Astudillo, Alma M. ; Pereira, Laura; Fernández-Caballero, Lidia; Balboa, María A. ; Balsinde, Jesús
KeywordsArachidonic acid
Mass spectrometry
Lipid signaling
Inflammation
Phospholipase A2
Monocytes/macrophages
Issue Date20-Aug-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationCells 8(8): 941 (2019)
AbstractHuman monocytes exposed to free arachidonic acid (AA), a secretory product of endothelial cells, acquire a foamy phenotype which is due to the accumulation of cytoplasmic lipid droplets with high AA content. Recruitment of foamy monocytes to the inflamed endothelium contributes to the development of atherosclerotic lesions. In this work, we investigated the potential role of AA stored in the neutral lipids of foamy monocytes to be cleaved by lipases and contribute to lipid mediator signaling. To this end, we used mass spectrometry-based lipidomic approaches combined with strategies to generate monocytes with different concentrations of AA. Results from our experiments indicate that the phospholipid AA pool in monocytes is stable and does not change upon exposure of the cells to the external AA. On the contrary, the AA pool in triacylglycerol is expandable and can accommodate relatively large amounts of fatty acid. Stimulation of the cells with opsonized zymosan results in the expected decreases of cellular AA. Under all conditions examined, all of the AA decreases observed in stimulated cells were accounted for by decreases in the phospholipid pool; we failed to detect any contribution of the triacylglycerol pool to the response. Experiments utilizing selective inhibitors of phospholipid or triacylglyerol hydrolysis confirmed that the phospholipid pool is the sole contributor of the AA liberated by stimulated cells. Thus, the AA in the triacylglycerol is not a source of free AA for the lipid mediator signaling during stimulation of human foamy monocytes and may be used for other cellular functions.
Publisher version (URL)https://doi.org/10.3390/cells8080941
URIhttp://hdl.handle.net/10261/188943
DOI10.3390/cells8080941
E-ISSN2073-4409
Appears in Collections:(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
neutral_lipids_source_arachidonic_acid_lipid_mediator_signaling.pdf2,29 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.