English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/18881
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 71 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Oxidative stress induced by long-term plum pox virus infection in peach (Prunus persica)

AutorHernández, José Antonio ; Rubio, Manuel ; Olmos, Enrique ; Ros Barceló, Alfonso; Martínez-Gómez, Pedro
Fecha de publicación25-nov-2004
EditorWiley-Blackwell
CitaciónPhysiologia Plantarum 122(4): 486-495 (2004)
ResumenIn this study, the effect of long-term plum pox virus (PPV) infection on the response of certain antioxidant enzymes at the subcellular level was studied in peach plants (Prunus persica (L.) Batch) (cv. GF305), which are characterized by great susceptibility to the virus. In infected plants, a decrease in the efficiency of excitation energy capture by PSII (Fv'/Fm') was observed, which was accompanied by a decrease in non-photochemical quenching (NPQ). p-Hydroxy-mercury benzoic acid (pHMB)-insensitive ascorbate peroxidase (APX) activity (class III peroxidase) was detected in both chloroplast and soluble fractions. In soluble fractions from inoculated peaches, a significant increase in pHMB-sensitive APX activity and a significant decrease in superoxide dismutase (SOD) activity were observed. These changes were correlated with the observations in isolated chloroplasts, where an increase in both pHMB-sensitive and pHMB-insensitive APX activities was observed, whereas significant decreases in SOD, monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) activities were produced. According to these results, as a consequence of PPV infection, an oxidative stress, indicated by an increase in lipid peroxidation and protein oxidation, was produced in peach leaves, which was monitored by the diaminobenzidine (DAB) peroxidase-coupled H2O2 probe. PPV infection produced an alteration in chloroplast ultrastructure, giving rise to dilated thylakoid membranes. PPV-infected peach leaves showed a decreased amount of starch in chloroplasts from palisade parenchyma, as well as an increase in the number and size of plastoglobuli, in relation to control plants. The results suggest that long-term PPV infection produces an oxidative stress, and that an antioxidative metabolism imbalance may be related to the progress of PPV infection and symptoms in peach plants.
Descripción10 pages, 4 figures, 4 tables.
Versión del editorhttp://dx.doi.org/10.1111/j.1399-3054.2004.00431.x
URIhttp://hdl.handle.net/10261/18881
DOI10.1111/j.1399-3054.2004.00431.x
ISSN0031-9317
Aparece en las colecciones: (CEBAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
prunus_persica_Hernandez.pdf387,64 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.