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Title

Development of a methodology for reversible chemical modification of silicon surfaces with application in nanomechanical biosensors

AuthorsSato, Roseli H.; Kosaka, Priscila M. ; Omori, Álvaro T.; Ferreira, Edgard A.; Petri, Denise F. S.; Malvar, Óscar ; Domínguez, Carmen M.; Pini, Valerio ; Ahumada, O.; Tamayo de Miguel, Francisco Javier ; Calleja, Montserrat ; Cunha, Rodrigo L. O. R.; Fiorito, Pablo A.
KeywordsBiosensor
Microcantilever
Chalcogen chemistry
Surface regeneration
Nanomechanical biosensors
Reversible immobilization
Issue Date15-Jul-2019
PublisherElsevier
CitationBiosensors and Bioelectronics 137: 287-293 (2019)
AbstractHypervalent tellurium compounds have a particular reactivity towards thiol compounds which are related to their biological properties. In this work, this property was assembled to tellurium-functionalized surfaces. These compounds were used as linkers in the immobilization process of thiolated biomolecules (such as DNA) on microcantilever surfaces. The telluride derivatives acted as reversible binding agents due to their redox properties, providing the regeneration of microcantilever surfaces and allowing their reuse for further biomolecules immobilizations, recycling the functional surface. Initially, we started from the synthesis of 4-((3-((4-methoxyphenyl) tellanyl) phenyl) amino)-4-oxobutanoic acid, a new compound, which was immobilized on a silicon surface. In nanomechanical systems, the detection involved a hybridization study of thiolated DNA sequences. Fluorescence microscopy technique was used to confirm the immobilization and removal of the telluride-DNA system and provided revealing results about the potentiality of applying redox properties to chalcogen derivatives at surfaces.
Publisher version (URL)https://doi.org/10.1016/j.bios.2019.04.028
URIhttp://hdl.handle.net/10261/188767
DOI10.1016/j.bios.2019.04.028
ISSN0956-5663
Appears in Collections:(IMN-CNM) Artículos
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