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dc.contributor.authorAlfonso Lozano, Miguel-
dc.contributor.authorCollados Collados, Raquel-
dc.contributor.authorYruela Guerrero, Inmaculada-
dc.contributor.authorPicorel Castaño, Rafael-
dc.date.accessioned2009-11-19T14:46:55Z-
dc.date.available2009-11-19T14:46:55Z-
dc.date.issued2004-07-
dc.identifier.citationPlanta, Volume 219, Number 3 / julio de 2004: 428-439.en_US
dc.identifier.issn0032-0935-
dc.identifier.urihttp://hdl.handle.net/10261/18834-
dc.descriptionThe definitive version is available at: http://www.springerlink.com/content/100484/en_US
dc.description.abstractPhotoinhibition and recovery were studied in two photosynthetic cell suspensions from soybean (Glycine max L. Merr): the wild type (WT) and the herbicide-resistant D1 mutant STR7. This mutant also showed an increase in saturated fatty acids from thylakoid lipids. STR7 was more sensitive to photoinhibition under culture conditions. In vivo photoinhibition experiments in the presence of chloramphenicol, in vitro studies in isolated thylakoid membranes, and immunoblot analysis indicated that the process of light-induced degradation of the D1 protein was not involved in the response of STR7 to light. At growth temperature (24°C), the recovery rate of photoinhibited photosystem II (PSII) was slower in STR7 relative to WT. Photoinhibition and recovery were differentially affected by temperature in both cell lines. The rates of photoinhibition were faster in STR7 at any temperature below 27°C. The rates of PSII recovery from STR7 were more severely affected than those of WT at temperatures lower than 24°C. The photoinhibition and recovery rates of WT at 17°C mimicked those of STR7 at 24°C. In organelle translation studies indicated that synthesis and elongation of D1 were substantially similar in both cell lines. However, sucrose gradient fractionation of chloroplast membranes demonstrated that D1 and also other PSII proteins such as D2, OEE33, and LCHII had a reduced capability to incorporate into PSII to yield a mature assembled complex in STR7. This effect may become the rate-limiting step during the recovery of photoinhibited PSII and may explain the increased sensitivity to high light found in STR7. Our data may hint at a possible role of fatty acids from membrane lipids in the assembly and dynamics of PSII.en_US
dc.description.sponsorshipThis work was supported by the MCYT (Grants PB98-1632 and BMC2002-00031). M.A. is the recipient of a contract from MCYT (Programa Ramón y Cajal) and R.C. is the recipient of a fellowship from Gobierno de Aragón (CONSI+D-DGA).en_US
dc.format.extent2597294 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsopenAccessen_US
dc.subjectCell cultureen_US
dc.subjectFatty acid unsaturationen_US
dc.subjectGlycineen_US
dc.subjectHerbicide resistanceen_US
dc.subjectLight stressen_US
dc.subjectPhotosystem IIen_US
dc.titlePhotoinhibition and recovery in a herbicide-resistant mutant from Glycine max (L.) Merr. cell cultures deficient in fatty acid unsaturationen_US
dc.typeartículoen_US
dc.identifier.doi10.1007/s00425-004-1242-5-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://www.springerlink.com/content/kuvgu10qd2rxbjem/fulltext.htmlen_US
dc.identifier.e-issn1432-2048-
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