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Título

Assessment of the potential skin irritation of lysine-derivative anionic surfactants using mouse fibroblasts and human keratinocytes as an alternative to animal testing

AutorSánchez, Lourdes; Mitjans, Montserrat; Infante, María Rosa; Vinardell, M. Pilar
Palabras claveCytotoxicity
Fibroblast
Keratinocyte
Lysine-derivative surfactants
Skin irritation
Fecha de publicación30-dic-2004
EditorSpringer
CitaciónPharmaceutical Research 21(9):1637-1641
ResumenPurpose. The aim of this study was to identify new surfactants with low skin irritant properties for use in pharmaceutical and cosmetic formulations, employing cell culture as an alternative method to in vivo testing. In addition, we sought to establish whether potential cytotoxic properties were related to the size of the counterions bound to the surfactants.
Methods. Cytotoxicity was assessed in the mouse fibroblast cell line 3T6 and the human keratinocyte cell line NCTC 2544 using the MTT assay and uptake of the vital dye neutral red 24 h after dosing (NRU).
Results. Lysine-derivative surfactants showed higher IC50s than did commercial anionic irritant compounds such as sodium dodecyl sulfate, proving to be no more harmful than amphoteric betaines. The aggressiveness of the surfactants depended on the size of their constituent counterions: surfactants associated with lighter counterions showed a proportionally higher aggressivity than those with heavier ones.
Conclusions. Synthetic lysine-derivative anionic surfactants are less irritant than commercial surfactants such as sodium dodecyl sulfate and hexadecyltrimethylammonium bromide and are similar to betaines. These surfactants may offer promising applications in pharmaceutical and cosmetic preparations, representing a potential alternative to commercial anionic surfactants as a result of their low irritancy potential.
Descripción5 pages, 3 figures, 1 table.-- PMID: 15497690 [PubMed].-- Print version published Sep 2004.
Versión del editorhttp://dx.doi.org/10.1023/B:PHAM.0000041459.63362.6f
URIhttp://hdl.handle.net/10261/18776
DOI10.1023/B:PHAM.0000041459.63362.6f
ISSN0724-8741 (Print)
1573-904X (Online)
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