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dc.contributor.authorVázquez, Lucíaes_ES
dc.contributor.authorFlórez García, Ana Belénes_ES
dc.contributor.authorMayo Pérez, Baltasares_ES
dc.identifier.citation7th Congress of European Microbiologists FEMS (2017)es_ES
dc.descriptionTrabajo presentado en el 7th Congress of European Microbiologists (FEMS 2017), celebrado en Valencia (España), del 9 al 13 de julio de 2017es_ES
dc.description.abstract[Backgrounds] High intake of soy or purified isoflavones has been associated with less intense menopause symptoms and a reduced risk of hormone-mediated diseases. Isoflavones from the diet can be metabolized into fully-active compounds or inactive metabolites by bacteria from the human gastrointestinal tract, which at present are poorly characterized. [Objectives] In this work, we assessed the metabolism of the two majority soy isoflavones (daidzein and genistein) by cultures of faecal slurries from menopausal woman with a positive or negative equol-production phenotype. [Methods] Faecal samples of five women were homogenized and inoculated in a general medium for colon bacteria. Faecal homogenates, bulk cultures and isolated colonies from the counting plates were incubated in GAM+0.5% arginine supplemented with 100 mM daidzein or genistein. Isoflavones and their metabolites in the cultures were analysed by UHPLC. In addition, microbial DNA was purified and used in conventional and real-time PCR analysis in order to identify and quantify specific genes involved in equol formation from daidzein. [Conclusions] Equol was only present in cultures derived from equol-producing women inoculated with homogenates and bulk colonies from the counting plates. Variable amounts of genistein and dihydrogenistein were scored when this isoflavone was added. Both ddr and tdr genes, encoding reductases involved in equol production, were detected in similar amounts in DNA from faeces and cultures thereof of equol-producing women. Occasionally, these genes were also correlated with genistein-derived metabolites. The biological significance of the presence/absence of tdr and ddr in isoflavone metabolism is currently under study.es_ES
dc.titleMetabolism of soy isoflavones by bacteria of intestinal origines_ES
dc.typepóster de congresoes_ES
dc.description.peerreviewedPeer reviewedes_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidMayo Pérez, Baltasar [0000-0001-5634-6543]es_ES
Appears in Collections:(IPLA) Comunicaciones congresos
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