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http://hdl.handle.net/10261/187050
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dc.contributor.author | Castro-Smirnov, Fidel Antonio | - |
dc.contributor.author | Piétrement, Olivier | - |
dc.contributor.author | Aranda, Pilar | - |
dc.contributor.author | Bertrand, Jean-Rémi | - |
dc.contributor.author | Ayache, Jeanne | - |
dc.contributor.author | Cam, Eric le | - |
dc.contributor.author | Ruiz-Hitzky, Eduardo | - |
dc.contributor.author | Lopez, Bernard S. | - |
dc.date.accessioned | 2019-07-29T07:51:11Z | - |
dc.date.available | 2019-07-29T07:51:11Z | - |
dc.date.issued | 2016-11-03 | - |
dc.identifier | doi: 10.1038/srep36341 | - |
dc.identifier | e-issn: 2045-2322 | - |
dc.identifier.citation | Scientific Reports 6: 36341 (2016) | - |
dc.identifier.uri | http://hdl.handle.net/10261/187050 | - |
dc.description.abstract | Nanofibers of sepiolite, a natural silicate belonging to the clay minerals family, might constitute a potential promising nanocarrier for the non-viral transfer of bio-molecules. We show here that sepiolite nanofibers efficiently bind different types of DNA molecules through electrostatic interactions, hydrogen bonding, cation bridges, and van der Waals forces. Moreover, Fourier-transform infrared spectroscopy identified the external silanol groups as the main sites of interaction with the DNA. Furthermore, as a proof of concept, we show that sepiolite is able to stably transfer plasmid DNA into mammalian cells and that the efficiency can be optimized. Indeed, sonication of sepiolite 100-fold stimulated DNA transfection efficiency. These results open the way to the use of sepiolite-based biohybrids as a novel class of nanoplatform for gene transfer with potential clinical applications. | - |
dc.description.sponsorship | This work was supported by La Ligue Nationale Contre le Cancer, ANR (Agence Nationale de la Recherche, ANR-14-CE10-0010-02), AFM-Téléthon and INCa (Institut National du Cancer, 2011-1-RT-01, 2011-1-PLBIO-09, 2013-1-PLBIO-14), the CICYT, Spain (projects MAT2012-31759 & MAT2015-71117-R) and the EU COST Action MP1202. | - |
dc.publisher | Springer Nature | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-71117-R | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1038/srep36341 | - |
dc.description.peerreviewed | Peer Reviewed | - |
dc.relation.publisherversion | https://doi.org/10.1038/srep36341 | - |
dc.date.updated | 2019-07-29T07:51:11Z | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | European Cooperation in Science and Technology | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Institut National du Cancer (France) | - |
dc.contributor.funder | Ligue Nationale contre le Cancer (France) | - |
dc.contributor.funder | Agence Nationale de la Recherche (France) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000921 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001665 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004099 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006364 | es_ES |
dc.identifier.pmid | 27808269 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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physical_interactions_DNA_sepiolite_nanofibers_transfer_mammalian_cells.pdf | 1,71 MB | Adobe PDF | Visualizar/Abrir |
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