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Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

AuthorsCastro-Smirnov, Fidel Antonio; Piétrement, Olivier; Aranda, Pilar CSIC ORCID ; Bertrand, Jean-Rémi; Ayache, Jeanne; Cam, Eric le; Ruiz-Hitzky, Eduardo CSIC ORCID; Lopez, Bernard S.
Issue Date3-Nov-2016
PublisherSpringer Nature
CitationScientific Reports 6: 36341 (2016)
AbstractNanofibers of sepiolite, a natural silicate belonging to the clay minerals family, might constitute a potential promising nanocarrier for the non-viral transfer of bio-molecules. We show here that sepiolite nanofibers efficiently bind different types of DNA molecules through electrostatic interactions, hydrogen bonding, cation bridges, and van der Waals forces. Moreover, Fourier-transform infrared spectroscopy identified the external silanol groups as the main sites of interaction with the DNA. Furthermore, as a proof of concept, we show that sepiolite is able to stably transfer plasmid DNA into mammalian cells and that the efficiency can be optimized. Indeed, sonication of sepiolite 100-fold stimulated DNA transfection efficiency. These results open the way to the use of sepiolite-based biohybrids as a novel class of nanoplatform for gene transfer with potential clinical applications.
Publisher version (URL)https://doi.org/10.1038/srep36341
Identifiersdoi: 10.1038/srep36341
e-issn: 2045-2322
Appears in Collections:(ICMM) Artículos
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