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Title

Vitamin D differentially regulates colon stem cells in patient-derived normal and tumor organoids

AuthorsFernández-Barral, A. ; Costales-Carrera, Alba; Buira, Sandra P.; Jung, Peter; Ferrer-Mayorga, Gemma ; Larriba, María Jesús ; Bustamante-Madrid, Pilar; Domínguez, Orlando; Real, Francisco X.; Guerra-Pastrián, Laura; Lafarga, Miguel; García-Olmo, Damián; Cantero, Ramón; Peso, Luis del; Batlle, Eduard; Rojo, Federico; Muñoz Terol, Alberto ; Barbáchano, Antonio
KeywordsColon cancer
Colon stem cells
Organoids
Stemness genes
Vitamin D
Issue Date2019
PublisherJohn Wiley & Sons
CitationFEBS Journal: (2019)
AbstractIntestine is a major target of vitamin D and several studies indicate an association between vitamin D deficiency and inflammatory bowel diseases (IBD), but also increased colorectal cancer (CRC) risk and mortality. However, the putative effects of 1α,25-dihydroxyvitamin D3 (calcitriol), the active vitamin D metabolite, on human colonic stem cells are unknown. Here we show by immunohistochemistry and RNAscope in situ hybridization that vitamin D receptor (VDR) is unexpectedly expressed in LGR5+ colon stem cells in human tissue and in normal and tumor organoid cultures generated from patient biopsies. Interestingly, normal and tumor organoids respond differentially to calcitriol with profound and contrasting changes in their transcriptomic profiles. In normal organoids calcitriol upregulates stemness-related genes, such as LGR5, SMOC2, LRIG1, MSI1, PTK7 and MEX3A, and inhibits cell proliferation. In contrast, in tumor organoids calcitriol has little effect on stemness-related genes while it induces a differentiated phenotype, and variably reduces cell proliferation. Concordantly, electron microscopy showed that calcitriol does not affect the blastic undifferentiated cell phenotype in normal organoids but it induces a series of differentiated features in tumor organoids. Our results constitute the first demonstration of a regulatory role of vitamin D on human colon stem cells, indicating a homeostatic effect on colon epithelium with relevant implications in IBD and CRC.
DescriptionIn Press.
Publisher version (URL)https://doi.org/10.1111/febs.14998
URIhttp://hdl.handle.net/10261/186482
DOI10.1111/febs.14998
ISSN1742-464X
E-ISSN1742-4658
Appears in Collections:(IIBM) Artículos
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