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Electrocatalytic processes promoted by diamond nanoparticles in enzymatic biosensing devices

AuthorsBriones, M.; Petit-Domínguez, M. D.; Parra-Alfambra, A. M.; Vázquez, Luis ; Pariente, F.; Lorenzo, E.; Casero, E.
KeywordsDiamond nanoparticles
Lactate determination
Electrochemical biosensors
Issue DateOct-2016
CitationBioelectrochemistry 111: 93-99 (2016)
AbstractWe have developed a biosensing platform for lactate determination based on gold electrodes modified with diamond nanoparticles of 4 nm of nominal diameter, employing the enzyme lactate oxidase and (hydroxymethyl)ferrocene (HMF) as redox mediator in solution. This system displays a response towards lactate that is completely different to those typically observed for lactate biosensors based on other nanomaterials, such as graphene, carbon nanotubes, gold nanoparticles or even diamond nanoparticles of greater size. We have observed by cyclic voltammetry that, under certain experimental conditions, an irreversible wave (E = +0.15 V) appears concomitantly with the typical Fe/Fe peaks (E = +0.30 V) of HMF. In this case, the biosensor response to lactate shows simultaneous electrocatalytic peaks at +0.15 V and +0.30 V, indicating the concurrence of different feedback mechanisms. The achievement of a biosensor response to lactate at +0.15 V is very convenient in order to avoid potential interferences. The developed biosensor presents a linear concentration range from 0.02 mM to 1.2 mM, a sensitivity of 6.1 μA mM, a detection limit of 5.3 μM and excellent stability. These analytical properties compare well with those obtained for other lactate-based biosensors that also include nanomaterials and employ HMF as redox mediator.
Publisher version (URL)https://doi.org/10.1016/j.bioelechem.2016.05.007
Identifiersdoi: 10.1016/j.bioelechem.2016.05.007
issn: 1567-5394
Appears in Collections:(ICMM) Artículos
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