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Title

DNA methylation levels of sex-related genes involved in the gonadal development of zebrafish (Danio rerio)

AuthorsValdivieso, A. CSIC ORCID; Anastasiadi, Dafni CSIC ORCID; Ribas, Laia CSIC ORCID ; Piferrer, Francesc CSIC ORCID
Issue Date16-Mar-2018
PublisherSocietat Catalana de Biologia
CitationVIII Jornada de Cromatina i Epigenètica (2018)
AbstractFish exhibit all types of reproduction systems known in vertebrates. In fish, gonadal fate is the combined result of genetic and environmental influences and some evidence indicates that epigenetic processes such as DNA methylation may have an important role. However, little is known regarding the relationship between DNA methylation and the expression of key genes involved in sexual development. In this study, we analyzed the DNA methylation of the promoter regions of canonical reproduction-related genes (dmrt1, dmrt3a, amh, cyp19a1a, fshr, foxl2, ar, sf1 and fancl) and its association with sex in 90-day-old adults of two different AB zebrafish families. DNA was extracted from their gonads and NGS libraries were prepared and sequenced using a targeted bisulfite sequencing approach. Results showed that there were significantly higher DNA methylation levels in the promoter of amh, cyp19a1a, and foxl2 of males compared to females, while dmrt1 and dmrt3 a exhibited the opposite pattern. Principal Component Analysis (PCA) showed that gonadal samples could be resolved according to sex. However, a subset of males was grouped closer to females than to the rest of males, suggesting that these males were neomales (genetic females developing as phenotypic males) produced spontaneously by perturbations of the process of sexual differentiation with a yet unknown etiology. In order to further analyze these findings, we are currently analyzing the expression levels of key genes related with male and female differentiation. This study contributes to our understanding of the role of DNA methylation in gene expression regulation during critical steps of gonadal development in a vertebrate model
DescriptionVIII Jornada de Cromatina i Epigenètica organizada por la Secció de Cromatina i Epigenètica de la Societat Catalana de Biologia (SCB) con el Barcelona Chromatin Club (BCC), 16 March 2018, Barcelona.-- 1 page
URIhttp://hdl.handle.net/10261/185941
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