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dc.contributor.authorRios-Covián, Davides_ES
dc.contributor.authorNogacka, Alicjaes_ES
dc.contributor.authorSalazar, Nuriaes_ES
dc.contributor.authorHernández-Barranco, Ana Maríaes_ES
dc.contributor.authorCuesta Suárez, Isabeles_ES
dc.contributor.authorGueimonde Fernández, Migueles_ES
dc.contributor.authorGonzález de los Reyes-Gavilán, Claraes_ES
dc.date.accessioned2019-07-11T07:42:23Z-
dc.date.available2019-07-11T07:42:23Z-
dc.date.issued2018-01-03-
dc.identifier.citationCanadian Journal of Microbiology 64(3): 215-221 (2018)es_ES
dc.identifier.issn0008-4166-
dc.identifier.urihttp://hdl.handle.net/10261/185894-
dc.description.abstractMechanistic features that characterize the interaction and inhibition of the food-borne pathogen Listeria monocytogenes by members of the genus Bifidobacterium still remain unclear. In the present work, we tried to shed light on the influence that co-cultivation of L. monocytogenes with Bifidobacterium breve may exert on both microorganisms and on virulence of the pathogen. Production of acetate and lactate was measured by gas chromatography and high-performance liquid chromatography, respectively; bacterial counts were obtained by plate count; gene expression was determined by RT-qPCR; and haemolytic activity was analyzed against goat erythrocytes. We found slightly but significantly lower final counts of Listeria and Bifidobacterium (p < 0.05) and lower haemolytic efficiency in L. monocytogenes cells from cocultures than in those from monocultures. In contrast, the hly and luxS genes, which code for the cytolysin listeriolysin O and participate in biofilm formation, respectively, were overexpressed when L. monocytogenes was grown in coculture. This indicates that the presence of Bifidobacterium is able to modify the gene expression and haemolytic activity of L. monocytogenes when both microorganisms grow together.es_ES
dc.language.isoenges_ES
dc.publisherCanadian Science Publishinges_ES
dc.rightsclosedAccesses_ES
dc.subjectBifidobacteriumes_ES
dc.subjectListeria monocytogeneses_ES
dc.subjectVirulencees_ES
dc.subjectLuxSes_ES
dc.subjectListeriolysin Oes_ES
dc.titleBifidobacterium breve IPLA20005 affects in vitro the expression of hly and luxs genes, related to the virulence of listeria monocytogenes Lm23es_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1139/cjm-2017-0625-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1139/cjm-2017-0625es_ES
dc.identifier.e-issn1480-3275-
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidRios-Covián, David [0000-0002-3639-0050]es_ES
dc.contributor.orcidNogacka, Alicja [0000-0001-8300-6149]es_ES
dc.contributor.orcidSalazar, Nuria [0000-0003-1435-7628]es_ES
dc.contributor.orcidHernández-Barranco, Ana María [0000-0002-3086-2563]es_ES
dc.contributor.orcidGueimonde Fernández, Miguel [0000-0002-0192-901X]es_ES
dc.contributor.orcidGonzález de los Reyes-Gavilán, Clara [0000-0001-9396-631]es_ES
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