English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/185894
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Bifidobacterium breve IPLA20005 affects in vitro the expression of hly and luxs genes, related to the virulence of listeria monocytogenes Lm23

AuthorsRios-Covián, David ; Nogacka, Alicja; Salazar, Nuria ; Hernández-Barranco, Ana María ; Cuesta Suárez, Isabel ; Gueimonde Fernández, Miguel ; González de los Reyes-Gavilán, Clara
Listeria monocytogenes
Listeriolysin O
Issue Date3-Jan-2018
PublisherCanadian Science Publishing
CitationCanadian Journal of Microbiology 64(3): 215-221 (2018)
AbstractMechanistic features that characterize the interaction and inhibition of the food-borne pathogen Listeria monocytogenes by members of the genus Bifidobacterium still remain unclear. In the present work, we tried to shed light on the influence that co-cultivation of L. monocytogenes with Bifidobacterium breve may exert on both microorganisms and on virulence of the pathogen. Production of acetate and lactate was measured by gas chromatography and high-performance liquid chromatography, respectively; bacterial counts were obtained by plate count; gene expression was determined by RT-qPCR; and haemolytic activity was analyzed against goat erythrocytes. We found slightly but significantly lower final counts of Listeria and Bifidobacterium (p < 0.05) and lower haemolytic efficiency in L. monocytogenes cells from cocultures than in those from monocultures. In contrast, the hly and luxS genes, which code for the cytolysin listeriolysin O and participate in biofilm formation, respectively, were overexpressed when L. monocytogenes was grown in coculture. This indicates that the presence of Bifidobacterium is able to modify the gene expression and haemolytic activity of L. monocytogenes when both microorganisms grow together.
Publisher version (URL)http://dx.doi.org/10.1139/cjm-2017-0625
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,35 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.