English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/185079
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Characterization of the European Sea Bass (Dicentrarchus labrax) Gonadal Transcriptome During Sexual Development

AuthorsRibas, Laia ; Crespo, Berta ; Sánchez Baizán, Núria ; Xavier, D.; Kuhl, Heiner; Rodríguez, J. M.; Díaz, Noelia ; Boltaña, Sebastian; MacKenzie, Simon; Moran, F.; Zanuy, Silvia ; Gómez, Ana ; Piferrer, Francesc
Sex differentiation
Issue DateJun-2019
CitationMarine Biotechnology 21(3): 359–373 (2019)
AbstractThe European sea bass is one of the most important cultured fish in Europe and has a marked sexual growth dimorphism in favor of females. It is a gonochoristic species with polygenic sex determination, where a combination between still undifferentiated genetic factors and environmental temperature determines sex ratios. The molecular mechanisms responsible for gonadal sex differentiation are still unknown. Here, we sampled fish during the gonadal developmental period (110 to 350 days post fertilization, dpf), and performed a comprehensive transcriptomic study by using a species-specific microarray. This analysis uncovered sex-specific gonadal transcriptomic profiles at each stage of development, identifying larger number of differentially expressed genes in ovaries when compared to testis. The expression patterns of 54 reproduction-related genes were analyzed. We found that hsd17β10 is a reliable marker of early ovarian differentiation. Further, three genes, pdgfb, snx1, and nfy, not previously related to fish sex differentiation, were tightly associated with testis development in the sea bass. Regarding signaling pathways, lysine degradation, bladder cancer, and NOD-like receptor signaling were enriched for ovarian development while eight pathways including basal transcription factors and steroid biosynthesis were enriched for testis development. Analysis of the transcription factor abundance showed an earlier increase in females than in males. Our results show that, although many players in the sex differentiation pathways are conserved among species, there are peculiarities in gene expression worth exploring. The genes identified in this study illustrate the diversity of players involved in fish sex differentiation and can become potential biomarkers for the management of sex ratios in the European sea bass and perhaps other cultured species
Description15 pages, 6 figures, supplementary material https://doi.org/10.1007/s10126-019-09886-x
Publisher version (URL)https://doi.org/10.1007/s10126-019-09886-x
Identifiersdoi: 10.1007/s10126-019-09886-x
issn: 1436-2228
e-issn: 1436-2236
Appears in Collections:(IATS) Artículos
(ICM) Artículos
Files in This Item:
File Description SizeFormat 
Ribas_et_al_2019_preprint.pdf1,34 MBAdobe PDFThumbnail
Ribas_et_al_2019_suppl.pdf812,25 kBAdobe PDFThumbnail
Ribas_et_al_2019_table_1.xlsx2,72 MBMicrosoft Excel XMLView/Open
Ribas_et_al_2019_table_2.xlsx90,33 kBMicrosoft Excel XMLView/Open
Ribas_et_al_2019_table_3.xls82 kBMicrosoft ExcelView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.