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Unraveling Sugar Binding Modes to DC-SIGN by Employing Fluorinated Carbohydrates

AuthorsMartínez, J. Daniel; Valverde, Pablo; Delgado, Sandra; Romanò, Cecilia; Linclau, Bruno; Reichardt, Niels C.; Oscarson, Stefan; Ardá, Ana ; Jiménez-Barbero, Jesús ; Cañada, F. Javier
Issue Date25-Jun-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationMolecules 24 (12): 2337 (2019)
AbstractA fluorine nuclear magnetic resonance (<sup>19</sup>F-NMR)-based method is employed to assess the binding preferences and interaction details of a library of synthetic fluorinated monosaccharides towards dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN), a lectin of biomedical interest, which is involved in different viral infections, including HIV and Ebola, and is able to recognize a variety of self- and non-self-glycans. The strategy employed allows not only screening of a mixture of compounds, but also obtaining valuable information on the specific sugar&ndash;protein interactions. The analysis of the data demonstrates that monosaccharides Fuc, Man, Glc, and Gal are able to bind DC-SIGN, although with decreasing affinity. Moreover, a new binding mode between Man moieties and DC-SIGN, which might have biological implications, is also detected for the first time. The combination of the <sup>19</sup>F with standard proton saturation transfer difference (<sup>1</sup>H-STD-NMR) data, assisted by molecular dynamics (MD) simulations, permits us to successfully define this new binding epitope, where Man coordinates a Ca<sup>2+</sup> ion of the lectin carbohydrate recognition domain (CRD) through the axial OH-2 and equatorial OH-3 groups, thus mimicking the Fuc/DC-SIGN binding architecture.
Identifiersdoi: 10.3390/molecules24122337
Appears in Collections:Colección MDPI
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