English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/184621
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Development and validation of a multianalyte immunoassay for the quantification of environmental pollutants in seawater samples from the Catalonia coastal area

AuthorsSanchis, Ana ; Bosch-Orea, Cristina ; Salvador, Juan Pablo ; Marco, María Pilar ; Farré, Marinella
Multianalyte immunoassay
Environmental analysis
Issue Date19-Jun-2019
PublisherSpringer Nature
CitationAnalytical and Bioanalytical Chemistry (2019)
AbstractFive different enzyme-linked immunosorbent assays (ELISAs) have been developed and applied for the detection of five representatives of important families of chemical pollutants in seawater: Irgarol 1051® (triazine biocide), sulfapyridine and chloramphenicol (antibiotics), 17β-estradiol (hormone), and domoic acid (algae toxin). The assays were validated by high-performance liquid chromatography (HPLC) coupled with high-resolution mass spectrometry (HRMS) showing good correlation between both immunochemical and chemical techniques. A process of extraction and clean-up was added prior to the analysis based on solid-phase extraction (SPE). The multianalyte platform presented good specificity for each compound and adequate sensitivity, with limits of detection (LOD) after the SPE treatment of 0.124 ± 0.006, 0.969 ± 0.09, 0.20 ± 0.05, 1.11 ± 0.012, and 1.39 ± 0.09 ng L−1 for Irgarol 1051®, sulfapyridine, chloramphenicol, 17β-estradiol, and domoic acid, respectively. No matrix effects were noticed in working with the seawater extracts. Afterward, seawater samples from the Mediterranean Sea (coastal area of Catalonia) were analyzed by both techniques and only one sample presented one contaminant, 17β-estradiol, in the concentration of 0.011 ± 0.04 μg L−1.
Publisher version (URL)https://doi.org/10.1007/s00216-019-01971-3
Appears in Collections:(IDAEA) Artículos
Files in This Item:
File Description SizeFormat 
Development and validation of a multianalyte immunoassay.pdf Embargoed until June 19, 2020Artículo principal275,92 kBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.