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dc.contributor.authorMartín-Gutiérrez, Guillermoes_ES
dc.contributor.authorRodríguez Beltrán, Jerónimoes_ES
dc.contributor.authorRodríguez-Martínez, José-Manueles_ES
dc.contributor.authorCostas, Colomaes_ES
dc.contributor.authorAznar Martín, Javieres_ES
dc.contributor.authorPascual, Álvaroes_ES
dc.contributor.authorBlázquez Gómez, Jesúses_ES
dc.identifier.citationAntimicrobial Agents and Chemotherapy 60: 4252-4258 (2016)es_ES
dc.description.abstractEscherichia coli isolates carrying chromosomally encoded low-level-quinolone-resistant (LLQR) determinants are frequently found in urinary tract infections (UTIs). LLQR mutations are considered the first step in the evolutionary pathway producing high-level fluoroquinolone resistance. Therefore, their evolution and dissemination might influence the outcome of fluoroquinolone treatments of UTI. Previous studies support the notion that low urine pH decreases susceptibility to ciprofloxacin (CIP) in E. coli. However, the effect of the urinary tract physiological parameters on the activity of ciprofloxacin against LLQR E. coli strains has received little attention. We have studied the activity of ciprofloxacin under physiological urinary tract conditions against a set of well-characterized isogenic E. coli derivatives carrying the most prevalent chromosomal mutations (ΔmarR, gyrA-S83L, gyrA-D87N, and parC-S80R and some combinations). The results presented here demonstrate that all the LLQR strains studied became resistant to ciprofloxacin (according to CLSI guidelines) under physiological conditions whereas the control strain lacking LLQR mutations did not. Moreover, the survival of some LLQR E. coli variants increased up to 100-fold after challenge with a high concentration of ciprofloxacin under UTI conditions compared to the results seen with Mueller-Hinton broth. These selective conditions could explain the high prevalence of LLQR mutations in E. coli. Furthermore, our data strongly suggest that recommended methods for MIC determination produce poor estimations of CIP activity against LLQR E. coli in UTIs.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.titleUrinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia colies_ES
dc.description.peerreviewedPeer reviewedes_ES
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