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Title

Simple and Practical Multigram Synthesis of D-Xylonate Using a Recombinant Xylose Dehydrogenase

AuthorsIsrael Sánchez-Moreno; Raúl Benito-Arenas; Pilar Montero-Calle; Carmen Hermida; Eduardo García-Junceda; Alfonso Fernández-Mayoralas
KeywordsBiotransformations
enzyme catalysis
oxidation
carbohydrates
gaxilose
xylose detection
xylonate synthesis
Issue Date19-Jun-2019
PublisherACS Publications
CitationACS Omega, 2019, 46, 10593-10598
AbstractAn efficient multienzyme system for the preparative synthesis of d-xylonate, a chemical with versatile industrial applications, is described. The multienzyme system is based on d-xylose oxidation catalyzed by the xylose dehydrogenase from Calulobacter crescentus and the use of catalytic amounts of NAD+. The cofactor is regenerated in situ by coupling the reduction of acetaldehyde into ethanol catalyzed by alcohol dehydrogenase from Clostridium kluyveri. Excellent conversions (>95%) were obtained in a process that allows easy product isolation by simple evaporation of the volatile buffer and byproducts.
Publisher version (URL)https://pubs.acs.org/doi/10.1021/acsomega.9b01090
URIhttp://hdl.handle.net/10261/184435
DOI10.1021/acsomega.9b01090
Appears in Collections:(IQOG) Artículos
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