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Título: | Profile of P-glycoprotein distribution in the rat and its possible influence on the salbutamol intestinal absorption process |
Autor: | Valenzuela, Belén CSIC ORCID; Nácher, Amparo; Ruiz-Carretero, Purificación; Martín-Villodre, Adela; López-Carballo, Gracia; Barettino, Domingo CSIC | Palabras clave: | salbutamol verapamil intestinal absorption intestinal secretion P-glycoprotein (P-gp) expression Bioavailability mRNA reverse transcription-polymerase chain reaction (RT-PCR) Western blot |
Fecha de publicación: | 15-abr-2004 | Editor: | Japanese Pharmacological Society | Citación: | Journal of Pharmacological Sciences 93(6):1641-1648 (2004) | Resumen: | The intrinsic absorption of salbutamol in different intestinal segments of the rat was measured and related with the corresponding intestinal P-glycoprotein (P-gp) expression levels. The apparent absorption rate constants (ka, h-1) observed in each fraction by means of the in situ rat gut absorption method after perfusion of a 0.29-mM isotonic solution of salbutamol were used as absorption indexes. In a separate series of studies, a semiquantitative analysis of the mRNA expression of P-gp by means of polymerase chain reaction and Western blot with an antibody raised against the P-gp were also performed. The in situ ka values determined in the different segments (h-1) showed that the absorption is not homogeneous along the intestinal tract, that is, 0.499 ± 0.054 for colon, 0.474 ± 0.052 for the proximal segment, 0.345 ± 0.014 for the mean, and 0.330 ± 0.023 for the distal fraction. Addition of verapamil to the perfusion fluid did provide a better absorption of salbutamol in the distal segment. The analysis of the mRNA expression and levels of P-gp showed that the enzyme content in each section of the intestine was inversely related to salbutamol absorption | Descripción: | 8 pages, 2 figures, 2 tables.--PMID: 15124220 [PubMed] | Versión del editor: | http://dx.doi.org/10.1002/jps.20071 | URI: | http://hdl.handle.net/10261/18438 | DOI: | 10.1002/jps.20071 | ISSN: | 1347-8613 (Print) |
Aparece en las colecciones: | (IBV) Artículos |
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