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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/18394
Título

Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis.

AutorPérez-Jímenez, Raul; Kosuri, Pallav; Rodríguez-Larrea, David; Gavira Gallardo, J. A. ; Fernández Soler, Julio M.; Sánchez-Ruiz, José M.; Wiita, Arun P.
Fecha de publicación3-oct-2008
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 283(40): 27121-27129 (2008)
ResumenUnderstanding how the catalytic mechanisms of enzymes are optimized through evolution remains a major challenge in molecular biology. The concept of co-evolution implicates that compensatory mutations occur to preserve the structure and function of proteins. We have combined statistical analysis of protein sequences with the sensitivity of single molecule force-clamp spectroscopy to probe how catalysis is affected by structurally distant correlated mutations in Escherichia coli thioredoxin. Our findings show that evolutionary anti-correlated mutations have an inhibitory effect on enzyme catalysis, whereas positively correlated mutations rescue the catalytic activity. We interpret these results in terms of an evolutionary tuning of both the enzyme-substrate binding process and the chemistry of the active site. Our results constitute a direct observation of distant residue co-evolution in enzyme catalysis.
Descripción22 pages, 6 figures, 1 table.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M803746200
URIhttp://hdl.handle.net/10261/18394
DOI10.1074/jbc.M803746200
ISSN0021-9258 (Print)
1083-351X (Online)
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