English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/183752
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria

AuthorsLapieza Remón, Mª Pilar; Jungas, Colette; Savirón, María; Jarne, Carmen; Membrado, Luis; Vela, Jesús ; Orduna, Jesús; Garriga, Rosa; Galbán, Javier; Cebolla, Vicente L.
KeywordsHPTLC-MS
Phospholipids
Electrospray mass spectrometry
Phospholipids identification
Issue Date4-Mar-2019
PublisherTaylor & Francis
CitationJournal of Liquid Chromatography and Related Technologies 42(1-2): 1-8 (2019)
AbstractHigh-performance thin-layer chromatography (HPTLC)-densitometry was directly combined with electrospray (ESI) tandem mass spectrometry for obtaining rapid and relevant structural identification of phospholipids (PL) species associated to membrane proteins (MP), in non-sulfur, purple bacteria having photosynthetic activity. Thus, species belonging to phosphatidylcholines (PC), phosphatidylethanolamines (PE), cardiolipins (CL) and phosphatidylglycerols (PG) associated to MP were investigated in bacterial membrane extracts from Rhodobacter (Rb.) blasticus, Rhodospirillum (R.) rubrum and Rhodobaca (Rbc.) bogoriensis, as well as those which are bound to a purified MP-photosynthetic complex from Rbc. bogoriensis. PL-classes were separated using a 7-step gradient-solvent sequence with a previous acid plate preconditioning, using Automated Multiple Development. Band zones of the plate corresponding to PL classes were selected to ensure their direct transfer to ion-trap MS equipment through an elution-based interface. Under the studied conditions, ESI+-MS spectra of PC and CL mostly showed sodium adducts ([M + Na]+) and [M-2H + 3Na]+, respectively, when recorded from the plate. The respective sodium adducts were fragmented in the ion-trap, and sodium remained as the charge of the fragment ions, thus being useful for their structural identification through MS/MS. ESI--MS and MS/MS spectra of CL were also obtained as [M-2H]2−, as well as those of PE and PG species as [M-H]- and [M]−, respectively. In this way, relative composition profiles of each studied PL-class by ESI-MS, and further identification of individual PL and the molecular species belonging to each of them by MS/MS were obtained.
Description8 Figuras.- 2 Tablas.- This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Liquid Chromatography and Related Technologies on 3th April 2019, available online: http://www.tandfonline.com/10.1080/10826076.2018.1561465
Publisher version (URL)http://dx.doi.org/10.1080/10826076.2018.1561465
URIhttp://hdl.handle.net/10261/183752
DOI10.1080/10826076.2018.1561465
ISSN1082-6076
E-ISSN1520-572X
Appears in Collections:(ICB) Artículos
(ICMA) Artículos
Files in This Item:
File Description SizeFormat 
LJLCRT_42_2019_HPTLC coupled.pdf Embargoed until March 4, 2020Artículo principal696,46 kBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.