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dc.contributor.authorGaugler, Stefanes_ES
dc.contributor.authorAl-Mazroua, Maha K.es_ES
dc.contributor.authorIssa, Sahar Y.es_ES
dc.contributor.authorRykl, Janaes_ES
dc.contributor.authorGrill, Matthiases_ES
dc.contributor.authorQanair, Asemes_ES
dc.contributor.authorCebolla, Vicente L.es_ES
dc.identifier.citationJournal of Analytical Toxicology 43(3): 212-220 (2019)es_ES
dc.description3 Figuras.- 3 Tablases_ES
dc.description.abstractIn this study, we describe the transfer of a new and fully automated workflow for the cost-effective drug screening of large populations based on the dried blood spot (DBS) technology. The method was installed at a routine poison control center and applied for DBS and dried urine spot (DUS) samples. A fast method focusing on the high-interest drugs and an extended screening method were developed on the automated platform. The dried cards were integrated into the automated workflow, in which the cards were checked in a camera recognition system, spiked with deuterated standards via an in-built spraying module and directly extracted. The extract was transferred online to an analytical LC column and then to the electrospray ionization tandem mass spectrometry system. The target compounds were analyzed in positive multiple-reaction monitoring mode. Before each sample batch or analysis day, calibration samples were measured to balance inter-day variations and to avoid false negative samples. An internal standard was integrated prior the sample extraction to allow in process control. A total of 28 target compounds were analyzed and directly extracted within 5 min per sample. This fast screening method was then extended to 20 min, enabling the usage of a Forensic Toxicology Database to screen over 1,200 drugs. The method gives confident positive/negative results for all tested drugs at their individual cut-off concentration. Good precision (±15%, respectively ±20% at limit of quantification) and correlation within the calibration range from 5 to 1,000 ng/mL was obtained. The method was finally applied to real cases from the lab and cross-checked with the existing methodologies.es_ES
dc.publisherOxford University Presses_ES
dc.subjectDrugs of abusees_ES
dc.subjectDrug screeninges_ES
dc.subjectDried blood spotes_ES
dc.subjectDried urine spotes_ES
dc.titleFully automated forensic routine dried blood spot screening for workplace testinges_ES
dc.description.peerreviewedPeer reviewedes_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidGaugler, Stefan [0000-0001-9059-0291]es_ES
dc.contributor.orcidIssa, Sahar Y. [0000-0001-5432-5028]es_ES
dc.contributor.orcidCebolla, Vicente L. [0000-0002-9786-9217]es_ES
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