English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/183654
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Fully automated forensic routine dried blood spot screening for workplace testing

AuthorsGaugler, Stefan; Al-Mazroua, Maha K.; Issa, Sahar Y.; Rykl, Jana; Grill, Matthias; Qanair, Asem; Cebolla, Vicente L.
KeywordsDrugs of abuse
Drug screening
Dried blood spot
Dried urine spot
Issue Date1-Oct-2018
PublisherOxford University Press
CitationJournal of Analytical Toxicology 43(3): 212-220 (2019)
AbstractIn this study, we describe the transfer of a new and fully automated workflow for the cost-effective drug screening of large populations based on the dried blood spot (DBS) technology. The method was installed at a routine poison control center and applied for DBS and dried urine spot (DUS) samples. A fast method focusing on the high-interest drugs and an extended screening method were developed on the automated platform. The dried cards were integrated into the automated workflow, in which the cards were checked in a camera recognition system, spiked with deuterated standards via an in-built spraying module and directly extracted. The extract was transferred online to an analytical LC column and then to the electrospray ionization tandem mass spectrometry system. The target compounds were analyzed in positive multiple-reaction monitoring mode. Before each sample batch or analysis day, calibration samples were measured to balance inter-day variations and to avoid false negative samples. An internal standard was integrated prior the sample extraction to allow in process control. A total of 28 target compounds were analyzed and directly extracted within 5 min per sample. This fast screening method was then extended to 20 min, enabling the usage of a Forensic Toxicology Database to screen over 1,200 drugs. The method gives confident positive/negative results for all tested drugs at their individual cut-off concentration. Good precision (±15%, respectively ±20% at limit of quantification) and correlation within the calibration range from 5 to 1,000 ng/mL was obtained. The method was finally applied to real cases from the lab and cross-checked with the existing methodologies.
Description3 Figuras.- 3 Tablas
Appears in Collections:(ICB) Artículos
Files in This Item:
File Description SizeFormat 
J ANAL TOXICOL 2018_43.pdfArtículo principal1,14 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.