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dc.contributor.authorGaugler, Stefan Christianes_ES
dc.contributor.authorRykl, Janaes_ES
dc.contributor.authorGrill, Matthiases_ES
dc.contributor.authorCebolla, Vicente L.es_ES
dc.date.accessioned2019-06-07T13:15:13Z-
dc.date.available2019-06-07T13:15:13Z-
dc.date.issued2018-01-
dc.identifier.citationJournal of Applied Bioanalysis 4(1): 7-15 (2018)es_ES
dc.identifier.issn2405-710X-
dc.identifier.urihttp://hdl.handle.net/10261/183652-
dc.description5 Figuras.- 3 Tablases_ES
dc.description.abstractA new and fully automated workflow for the cost effective drug screening of large populations based on the dried blood spot (DBS) technology was introduced in this study. DBS were prepared by spotting 15 μL of whole blood, previously spiked with alprazolam, amphetamine, cocaine, codeine, diazepam, fentanyl, lysergic acid diethylamide (LSD), 3,4-methylenedioxymethamphet-amine (MDMA), methadone, methamphetamine, morphine and oxycodone onto filter paper cards. The dried spots were scanned, spiked with deuterated standards and directly extracted. The extract was transferred online to an analytical LC column and then to the electrospray ionization tandem mass spectrometry system. All drugs were quantified at their cut-off level and good precision and correlation within the calibration range was obtained. The method was finally applied to DBS samples from two patients with back pain and codeine and oxycodone could be identified and quantified accurately below the level of misuse of 89.6 ng/mL and 39.6 ng/mL respectively.es_ES
dc.language.isoenges_ES
dc.publisherMedicina STM Editoreses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectDrugs of abusees_ES
dc.subjectDried blood spotes_ES
dc.subjectDBSes_ES
dc.subjectAutomationes_ES
dc.subjectTDMes_ES
dc.titleFully automated drug screening of dried blood spots using online LC-MS/MS analysises_ES
dc.typeartículoes_ES
dc.identifier.doi10.17145/jab.18.003-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.17145/jab.18.003es_ES
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidGaugler, Stefan Christian [0000-0001-9059-0291]es_ES
dc.contributor.orcidCebolla, Vicente L. [0000-0002-9786-9217]es_ES
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