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High-performance-TLC coupled to electrospray (ESI) ionization tandem mass spectrometry for identifying neutral lipids, sphingolipids and phospholipids in complex samples

AuthorsCebolla, Vicente L. ; Lapieza Remón, Mª Pilar; Jarne, Carmen; Savirón, María; Orduna, Jesús; Membrado, Luis; Morlock, Gertrud Elisabeth; Jungas, C.; Garriga, Rosa; Vela, Jesús; Galbán, Javier
Issue Date2017
CitationHPTLC 2017
AbstractHigh-performance thin-layer chromatography (HPTLC) is well-adapted to providing lipid-class separations, as an assistant technique of High-Performance Liquid Chromatography (HPLC). From this perspective, it can complement traditional LC-mass spectrometric approaches in a unique way. As the zones of interest on the plate can be selectively transferred to the ESI-MS instrument via an elution-based interface, a rapid, relevant structural information about molecular species within each lipid classes from complex samples can be obtained. Separation using AMD2 provides lipid-classes as narrow peaks enough to ensure a direct elution and transfer of the plate zones to obtain both composition profiles of each class by ESI-MS, and identification of individual lipids and molecular species belonging to each separated lipid class by MS/MS (MSn) and/or HR-MS. The respective sodium adducts of the above-mentioned lipid classes were fragmented in the positive ion mode using an ion-trap technology. The sodium remained the charge of their fragment ions, thus being useful for their structural identification by MS/MS (MSn) through further fragmentation. This work exemplarily focuses on profiling and identification of neutral lipids (NLs), sphingolipids (SLs) and phospholipids (PLs), in three analytical cases: (1) mono (MGs) and diacylglycerides (DGs) (in positive ion mode) and fatty acids (FAs) (in negative ion mode) as impurities in a fatty acid methyl ester (FAME)-based biodiesel sample; (2) molecular species of neutral sphingolipids (SL), such as sphingomyelins (SMs) and globotriaosylceramides (Gb3), in human plasma; (3) phosphatidylcholines (PCs), hosphatidylethanolamines (PEs), phosphatidyglicerols (PGs) and cardiolipines (CLs) associated to membrane proteins of photosynthetic purple bacteria.
DescriptionResumen del trabajo presentado al 23rd International Symposium for High-Performance Thin-Layer Chromatography (HPTLC), celebrado en Berlin (Alemania) del 4 al 8 de julio de 2017.
Appears in Collections:(ICMA) Comunicaciones congresos
(ICB) Comunicaciones congresos
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