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Interaction of antimicrobial arginine-based cationic surfactants with liposomes and lipid monolayers

AuthorsCastillo Expósito, José A.; Pinazo Gassol, Aurora ; Carilla Auguet, Josep ; Infante, María Rosa ; Alsina, M. Asunción ; Haro Villar, Isabel ; Clapés Saborit, Pere
KeywordsArginine-based cationic surfactants
Lipid Monolayers
1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)
1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] sodium salt (DPPG)
Issue Date13-Mar-2004
PublisherAmerican Chemical Society
CitationLangmuir 20(8): 3379-3387 (2004)
AbstractThe present work examines the relationship between the antimicrobial activity of novel arginine-based cationic surfactants and the physicochemical process involved in the perturbation of the cell membrane. To this end, the interaction of these surfactants with two biomembrane models, namely, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar lipid vesicles (MLVs) and monolayers of DPPC, 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] sodium salt (DPPG), and Escherichia coli total lipid extract, was investigated. For the sake of comparison, this study included two commercial antimicrobial agents, hexadecyltrimethylammonium bromide and chlorhexidine dihydrochloride. Changes in the thermotropic phase transition parameters of DPPC MLVs in the presence of the compounds were studied by differential scanning calorimetry analysis. The results show that variations in both the transition temperature (Tm) and the transition width at half-height of the heat absorption peak (ΔT1/2) were consistent with the antimicrobial activity of the compounds. Penetration kinetics and compression isotherm studies performed with DPPC, DPPG, and E. coli total lipid extract monolayers indicated that both steric hindrance effects and electrostatic forces explained the antimicrobial agent−lipid interaction. Overall, in DPPC monolayers single-chain surfactants had the highest penetration capacity, whereas gemini surfactants were the most active in DPPG systems. The compression isotherms showed an expansion of the monolayers compared with that of pure lipids, indicating an insertion of the compounds into the lipid molecules. Owing to their cationic character, they are incorporated better into the negatively charged DPPG than into zwitterionic DPPC lipid monolayers.
Description9 pages, 10 figures, 6 tables.-- PMID: 15875872 [PubMed].-- Printed version published Apr 13, 2004.-- Supporting information available at: http://pubs.acs.org/doi/suppl/10.1021/la036452h
Publisher version (URL)http://dx.doi.org/10.1021/la036452h
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