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Title

Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy

AuthorsGarcía, Pablo D.; García García, Ricardo
KeywordsContact mechanics
Force spectroscopy
Elastic properties
Cell nanomechanics
Issue Date2018
PublisherBiophysical Society
CitationBiophysical Journal 114: 2923- 2932 (2018)
AbstractThe elastic response of a living cell is affected by its physiological state. This property provides mechanical fingerprints of a cell's dysfunctionality. The softness (kilopascal range) and thickness (2–15 μm) of mammalian cells imply that the force exerted by the probe might be affected by the stiffness of the solid support. This observation makes infinite sample thickness models unsuitable to describe quantitatively the forces and deformations on a cell. Here, we report a general theory to determine the true Young's moduli of a single cell from a force-indentation curve. Analytical expressions are deduced for common geometries such as flat punches, paraboloids, cones, needles, and nanowires. For a given cell and indentation, the influence of the solid support on the measurements is reduced by using sharp and high aspect ratio tips. The theory is validated by finite element simulations.
Publisher version (URL)https://doi.org/10.1016/j.bpj.2018.05.012
URIhttp://hdl.handle.net/10261/183082
Identifiersdoi: 10.1016/j.bpj.2018.05.012
issn: 0006-3495
e-issn: 1542-0086
Appears in Collections:(ICMM) Artículos
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