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Título: | Determination of the viscoelastic properties of a single cell cultured on a rigid support by force microscopy |
Autor: | García, Pablo D.; García García, Ricardo CSIC ORCID | Palabras clave: | Viscoelastic theory Cell nanomechanics Force distance curves Atomic force microscopy Nanorheology Force spectroscopy |
Fecha de publicación: | 2018 | Editor: | Royal Society of Chemistry (UK) | Citación: | Nanoscale 10: 19799- 19809 (2018) | Resumen: | Understanding the relationship between the mechanical properties of living cells and physiology is a central issue in mechanobiology. Mechanical properties are used as fingerprints of the pathological state of a single cell. The force exerted on a cell is influenced by the stiffness of the solid support needed to culture it. This effect is a consequence of the cell's boundary conditions. It causes a cell to appear with mechanical properties different from their real values. Here we develop a bottom effect viscoelastic theory to determine the viscoelastic response of a cell. The theory transforms a force-distance curve into the cell's Young's modulus, loss modulus, relaxation time or viscosity coefficient with independence of the stiffness of the rigid support. The theory predicts that, for a given indentation, the force exerted on the cell's periphery will be larger than on a perinuclear region. Results based on the use of semi-infinite contact mechanics models introduce large numerical errors in the determination of the mechanical properties. Finite element simulations confirm the theory and define its range of applicability. | Versión del editor: | https://doi.org/10.1039/C8NR05899G | URI: | http://hdl.handle.net/10261/183081 | DOI: | 10.1039/c8nr05899g | Identificadores: | doi: 10.1039/c8nr05899g issn: 2040-3364 e-issn: 2040-3372 |
Aparece en las colecciones: | (ICMM) Artículos |
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Garcia_Bottom_effectViscoelasticTheory-Revised.docx | 1,13 MB | Microsoft Word XML | Visualizar/Abrir |
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