English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/183081
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Determination of the viscoelastic properties of a single cell cultured on a rigid support by force microscopy

AuthorsGarcía, Pablo D.; García García, Ricardo
KeywordsViscoelastic theory
Cell nanomechanics
Force distance curves
Atomic force microscopy
Force spectroscopy
Issue Date2018
PublisherRoyal Society of Chemistry (UK)
CitationNanoscale 10: 19799- 19809 (2018)
AbstractUnderstanding the relationship between the mechanical properties of living cells and physiology is a central issue in mechanobiology. Mechanical properties are used as fingerprints of the pathological state of a single cell. The force exerted on a cell is influenced by the stiffness of the solid support needed to culture it. This effect is a consequence of the cell's boundary conditions. It causes a cell to appear with mechanical properties different from their real values. Here we develop a bottom effect viscoelastic theory to determine the viscoelastic response of a cell. The theory transforms a force-distance curve into the cell's Young's modulus, loss modulus, relaxation time or viscosity coefficient with independence of the stiffness of the rigid support. The theory predicts that, for a given indentation, the force exerted on the cell's periphery will be larger than on a perinuclear region. Results based on the use of semi-infinite contact mechanics models introduce large numerical errors in the determination of the mechanical properties. Finite element simulations confirm the theory and define its range of applicability.
Publisher version (URL)https://doi.org/10.1039/C8NR05899G
Identifiersdoi: 10.1039/c8nr05899g
issn: 2040-3364
e-issn: 2040-3372
Appears in Collections:(ICMM) Artículos
Files in This Item:
File Description SizeFormat 
Garcia_Bottom_effectViscoelasticTheory-Revised.docx1,13 MBMicrosoft Word XMLView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.